Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Nucleotides are the building blocks for a variety of molecules, including ATP, which is broken down in the skeletal muscle cells to provide fuel for exercise. Nucleotides can be obtained from the diet, and produced or salvaged by the body. Though nucleotides provide the building blocks for ATP, the sugar ribose has been suggested to be rate limiting for synthesising ATP.
Previous studies have shown a decrease in ATP levels in the muscle after intense exercise, and this is linked to fatigue. Other studies have also reported benefits of nucleotide and/or ribose supplementation on exercise performance. However, any potential link between ATP metabolism and exercise performance has not been explored.
This study will look at the effects of nucleotide and nucleotide-ribose supplementation in fuel utilisation and performance in skeletal muscle during endurance exercise.
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Low nucleotide | Placebo Comparator | 2 week supplementation with a low nucleotide mycoprotein drink, with added dextrose |
|
| High nucleotide | Experimental | 2 week supplementation with a high nucleotide mycoprotein drink, with added dextrose |
|
| High nucletide + Ribose | Experimental | 2 week supplementation with a high nucleotide mycoprotein drink, with added ribose |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Low nucleotide | Dietary Supplement | 2 week, twice daily, supplementation with a nucleotide-depleted mycoprotein drink, with added dextrose |
|
| Measure | Description | Time Frame |
|---|---|---|
| Changes in muscle ATP concentrations | After each supplementation cycle (day 15 of each cycle): baseline (fasted and before exercise), immediately after glycogen depleting exercise (after exercising for 1:30 - 2 hours), immediately after 15 minute time trial and after 3-hour recovery |
| Measure | Description | Time Frame |
|---|---|---|
| Changes in muscle glycogen concentrations | After each supplementation cycle (day 15 of each cycle): baseline (fasted and before exercise), immediately after glycogen depleting exercise (after exercising for 1:30 - 2 hours), immediately after 15 minute time trial and after 3-hour recovery | |
| Blood lactate concentrations |
Not provided
Inclusion Criteria:
Exclusion Criteria:
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Exeter - St Luke's Campus | Exeter | Devon | EX2 4TH | United Kingdom |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| ID | Term |
|---|---|
| D059014 | High-Throughput Nucleotide Sequencing |
| D012266 | Ribose |
| ID | Term |
|---|---|
| D017421 | Sequence Analysis |
| D005821 | Genetic Techniques |
| D008919 | Investigative Techniques |
| D010429 | Pentoses |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| High nucleotide | Dietary Supplement | 2 week, twice daily, supplementation with a nucleotide-rich mycoprotein drink, with added dextrose |
|
| High nucletide + Ribose | Dietary Supplement | 2 week, twice daily, supplementation with a nucleotide-rich mycoprotein drink, with added ribose |
|
| After supplementation cycle (day 15): baseline and at 15 minute intervals up 1 hour during glycogen depleting exercise |
| Blood glucose concentrations | After supplementation cycle (day 15): baseline and at 15 minute intervals up 1 hour during glycogen depleting exercise |
| Serum uric acid concentrations | Measured at baseline and of days 6, 8 and 15 of each supplementation cycle |
| D009005 |
| Monosaccharides |
| D000073893 | Sugars |
| D002241 | Carbohydrates |