Increasing interest has emerged concerning ovarian tissue cryopreservation and subsequent transplantation as a potential way of preserving fertility in cancer patients as well as for women who just wish to delay childbearing. As the majority of existing protocols use the slow freezing method, this study aims to test vitrification techniques of ovarian cortex.

Inclusion Criteria:

• female-to-male transsexuals
• gender completing operation
• signed informed consent form
• willingness to donate ovarian tissue for research purposes
• age >=18 years

Exclusion Criteria:

• malignant disease
• age under 18 years
• refusal to donate ovarian tissue

female-to-male transsexuals