Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Class |
|---|---|
| INM : institut des neurosciences Montpellier | UNKNOWN |
Not provided
Not provided
Not provided
Not provided
Metalloproteinases (MMPs) constitute a family of endopeptidases that cleaves the extracellular matrix as collagen included in the eardrum. Activity of MMP has been shown in some otitis media with effusion (OME) fluids. These enzymes could be directly linked to the prosnostic of OME as it may damage the eardrum and leads to tympanic atrophy.
The main goal of the study is to find out the correlation between activity of MMPs and tympanic atrophy. The investigator will also study if there is any clinical predictive factors in relation to the level of MMPs.
The investigator will include 35 children under 12 years old with OME and who need tympanostomy or tube placement under general anesthesia. Middle ear effusion will be collected, divided in two and frozen.
The investigator will perform ELISA tests to measure the level of MMP2 MMP9 MMP7 et TIMP2 (inhibitor of MMPs). The investigator want to compare the level of MMPs between healthy eardrum and atrophic membrane.
Clinical data will be collected such as age, gender, chronicity of the OME, quality of the eardrum and middle ear effusion type to see if there is any difference when MMPs level is higher.
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Healthy eardrum | OME (otitis media with effusion)with normal tympanic membrane |
| |
| Atrophic eardrum | OME (otitis media with effusion) with atrophic membrane : thinning of the membrane, retraction pocket |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| preservation of mucosal otitis media | Other | Suction of the middle ear fluid and conservation of the fluid to be collected instead of thrown away into children Healthy eardrum and Atrophic eardrum |
| Measure | Description | Time Frame |
|---|---|---|
| Microscopic evaluation of the quality of the eardrum : normal or atrophic | Microscopic evaluation of the quality of the eardrum : normal or atrophic | before surgery |
| Measure | Description | Time Frame |
|---|---|---|
| biochemical assay of the level of TIMP | biochemical assay of the level of TIMP | 1 day |
| biochemical assay of the level of MMPs | biochemical assay of the level of MMPs |
Not provided
Inclusion Criteria:
- every children under 12 yo operated on in Gui de Chauliac Hospital
Exclusion criteria:
- when effusion is not possible to collect (too thick or amount of fluid not enough)
Collection of both MEE on each ear when OME is bilateral. We will perform the tests on only one ear. The other one will be kept in case of technical issue. A random draw will establish which ear will be tested.
Not provided
Not provided
Not provided
Children under the age of twelve with OME and surgical indication for myringostomy of tube placement
Not provided
| Name | Affiliation | Role |
|---|---|---|
| Lylou Casteil Baume, ENT resident | University Hospital, Montpellier | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Uhmontpellier | Montpellier | 34295 | France |
Not provided
| ID | Term |
|---|---|
| D010034 | Otitis Media with Effusion |
| ID | Term |
|---|---|
| D010033 | Otitis Media |
| D010031 | Otitis |
| D004427 | Ear Diseases |
| D010038 | Otorhinolaryngologic Diseases |
Not provided
Not provided
Not provided
Not provided
Not provided
Middle ear effusion fluids
| 1 day |