Nicotine Pharmacokinetic in Blood and Analysis of Exhaled Breath After E-cigarette Smoking.

Nicotine Pharmacokinetic Study and Volatile Organic Compound Analysis in Exhaled Breath After Electronic Cigarette Utilization.

Electronic or e-cigarettes are battery-powered devices that deliver vapor which may be inhaled in the manner tobacco is smoked. The e-liquid is usually a solution containing propylene glycol and/or vegetable glycerin, nicotine, and flavor concentrates, although some e-liquids also are sold as non-nicotine containing products. The long- and short-term public health consequences of e-cigarette use, including the effects of e-cigarettes on tobacco use behavior, are not well understood. However, the prevalence of e-cigarette use appears to be undergoing a rapid increase.

In this study the investigators propose to analyze nicotine pharmacokinetic in blood and to compare the exhaled breath before and after e-cigarette smoking. They want to obtain a pharmacokinetic model of this population for nicotine. And they want to identify if molecules contained in vapor are absorbed, metabolized or modified by the organism.

Inclusion Criteria:

• Subject who has already used electronic cigarette
• Subject who has been using electronic cigarette for more than one month and/or Tobacco smoker (traditional cigarettes)
• Subject without history of chronic pathology
• Subject not taking long-term drug treatment
• Subject able to understand studie's aims and risk

Exclusion Criteria:

• Subject with a chronic respiratory pathology
• Subject suffering from a respiratory or acute otorhinolaryngology pathology in progress or recent
• Subject under alcohol withdrawal
• Subject with heavy drinking or daily use of illegal drugs
• Subject with epilepsy
• Subject having hypersensitivity to one of the compounds of the spraying liquid
• Subject with liver failure
• Subject with severe renal impairment (GFR <60 ml / min)
• Subject with an allergy to the molecules contained in the e-liquid
• Subject suffering from claustrophobia
• Pregnancy or breastfeeding