Dental Restorations Effect on Oxidative Status of the Gingival Crevicular Fluid

Redox Interactions Between Dental Restorations and Gingival Crevicular Fluid

The goal of the study was to ascertain the influence of dental caries and teeth position as well as the type and amount of the applied dental materials (DMs) on the oxidative stress (OS) status in gingival crevicular fluid (GCF).

Six DMs were tested by investigators, each sealed in one session: amalgam (Amg), zinc phosphate cement (ZnPhC), zinc polycarboxylate cement (ZnPoC), glass ionomer cement (GIC) and composites, Tetric EvoCeram (TEC) and Beautifil (BF).

The study included 88 dental outpatients. Oxidative stress parameters, including malondialdehyde (MDA), glutathione (GSH) and total superoxide dismutase (tSOD) activity were measured in GCF, before (day 0) and after treatment (7th and 30th day).

Antagonistically positioned healthy teeth were used as a control.

Inclusion Criteria:

Criteria related to the condition of teeth:

• Presence of proximal caries on anterior and posterior teeth
• Existence of the antagonistic tooth ("mirror"-positioned healthy tooth) to be used as control
• An absence of fresh post-extraction or traumatic wounds in the restoration area or in the area of restored surfaces
• An absence of infection in the area of restored surfaces

Other influencing criteria:

• Absence of bone-associated diseases or treatments
• Satisfactory oral hygiene
• Patients exhibiting good compliance

Exclusion Criteria:

Criteria related to the condition of teeth:

• Endodontic and/or periodontal infections in the area of the cervical filling
• Presence of periodontopathy
• Prominent periodontal pockets
• Subgingival carries
• Prominent fillings outside the cavity

Other influencing criteria:

• Patients on radiation or immunosuppressive therapy
• Presence of bone-associated diseases and malignant diseases
• Patients using drugs/alcohol/cigarettes (> 20 cigarettes per day)
• Bad oral hygiene
• Patients exhibiting poor compliance