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Sarcopenia is an important component of cachexia associated with cancer, and their high incidence in cancer patients emphasizes the need for a better understanding of its mechanisms, which can result in better therapeutic interventions to reverse this situation and improve the prognosis. Our hypothesis is that the plasma concentration of IL-6 and c-terminal agrin is directly correlated with the loss of muscle mass and development of cachexia.
The agrin is a protein that acts on neuromuscular junctions (NMJs) promoting stabilization of same, which results in the maintenance and growth of muscle fibers, but the cleavage of agrin, a process by which is formed the agrin fragment C-terminus (CAF), has been linked to the development of sarcopenia, because its presence is directly linked to the reduction in the number of muscle fibers, increasing the heterogeneity of fiber size, presence of Central cores and increasing the proportion of type I fibers and consequently a greater degradation of lean body mass. Studies in mice show that the greatest cleavage of agrin carries on development of sarcopenia and human studies report that individuals with higher serum levels of sarcopenia CAF compared to individuals without sarcopenia.
Therefore, aiming at the complexity of cancer associated with the cachexia and the great importance of the maintenance of lean body mass to a better prognosis in disease, is of fundamental importance to elucidate the role of CAF and the factors associated with sarcopenia, the possible use of these proteins for diagnosis and the contribution that this clarification could bring in clinical therapy.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Group with cancer cachexia (CTB) | For diagnosis of cachexia it will be used the following criteria (Evans et al., 2008) | ||
| Group without cancer cachexia (TB) |
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| Measure | Description | Time Frame |
|---|---|---|
| Agrin fragment c-terminus CAF in cancer and cancer cachexia | To measure the contents of agrin fragment c-terminus (CAF) in plasma of patient with cancer and cancer cachexia. | 1 month |
| Agrin fragment c-terminus CAF in cancer sarcopenia | To analyze correlation between Agrin fragment c-terminus CAF and the lean body mass (CT-scan estimated) of patients with cancer and with cancer cachexia. | 1 month |
| Agrin fragment c-terminus CAF and IL-6 levels | To correlate levels of agrin fragment c-terminus (CAF) and IL-6 plasma levels in patients with cancer and with cancer cachexia. | 1 month |
| Agrin fragment c-terminus (CAF) and IL-6 and lean body mass | To correlate levels of agrin fragment c-terminus (CAF) and IL-6 with the lean body mass | 1 month |
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Inclusion Criteria:
Exclusion Criteria:
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Oure sample will be selected between cancer patient of Rome Umberto I Hospital
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Laviano Alessandro | Contact | +390649973902 | alessandro.laviano@uniroma1.it |
| Name | Affiliation | Role |
|---|---|---|
| Alessandro Laviano, MD | Department of Clinical Medicine, Sapienza University of Rome, Italy | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Department of Clinical Medicine, Sapienza University of Rome | Recruiting | Rome | 00185 | Italy |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 9276862 | Background | Argiles JM, Alvarez B, Lopez-Soriano FJ. The metabolic basis of cancer cachexia. Med Res Rev. 1997 Sep;17(5):477-98. doi: 10.1002/(sici)1098-1128(199709)17:53.0.co;2-r. No abstract available. | |
| 19713854 | Background | Argiles JM, Busquets S, Toledo M, Lopez-Soriano FJ. The role of cytokines in cancer cachexia. Curr Opin Support Palliat Care. 2009 Dec;3(4):263-8. doi: 10.1097/SPC.0b013e3283311d09. |
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We are still evaluate a researcher to analyze data collected
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| ID | Term |
|---|---|
| D002100 | Cachexia |
| D009369 | Neoplasms |
| D055948 | Sarcopenia |
| ID | Term |
|---|---|
| D015431 | Weight Loss |
| D001836 | Body Weight Changes |
| D001835 | Body Weight |
| D012816 | Signs and Symptoms |
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Blood samples: determination of: serum C-reactive protein, albumin, creatinine and haemoglobin concentrations. Agrin fragment c-terminus and IL-6 will be carried out using specific ELISA kits.
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| D013568 |
| Pathological Conditions, Signs and Symptoms |
| D013851 | Thinness |
| D009133 | Muscular Atrophy |
| D020879 | Neuromuscular Manifestations |
| D009461 | Neurologic Manifestations |
| D009422 | Nervous System Diseases |
| D001284 | Atrophy |
| D020763 | Pathological Conditions, Anatomical |