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This study will examine the effect of exercise on the turnover of collagen in articular cartilage and the content of cytokines in the synovial fluid from human adults with osteoarthritis.
By using stabile isotope labelling, this study will track the formation and destination of newly formed cartilage collagen in adult humans scheduled for knee replacement surgery due to osteoarthritis. The collagen formation as well as the effect on the cytokine content of the synovial fluid will be examined both at rest and in response to 4 weeks of exercise/training. Overall the project aims to improve the basic understanding of cartilage development and function in relation to osteoarthritis prevention.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Exercise group | Experimental |
| |
| Control group | No Intervention |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Exercise. | Behavioral | Moderate to heavy intensity resistance exercise consisting of leg press. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Collagen synthesis in articular cartilage | By using incorporation of stabile isotope tracers measured by gas chromatography, the concentration of newly formed collagen will be measured. | End of study at 4 weeks |
| Measure | Description | Time Frame |
|---|---|---|
| Cytokine and enzymes concentration in synovial fluid | By using Enzyme-Linked Immunosorbent Assay (ELISA) the concentration of cytokines in synovial fluid will be measured. | End of study at 4 weeks |
| Cytokine and enzymes concentration in blood |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Adam EM Jørgensen, MD | Institute of Sports Medicine, Copenhagen | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Institute of Sports Medicine Copenhagen | Copenhagen | 2400 | Denmark |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 36720425 | Derived | Jorgensen AEM, Schjerling P, DellaValle B, Rungby J, Kjaer M. Acute loading has minor influence on human articular cartilage gene expression and glycosaminoglycan composition in late-stage knee osteoarthritis: a randomised controlled trial. Osteoarthritis Cartilage. 2023 Jul;31(7):884-893. doi: 10.1016/j.joca.2023.01.317. Epub 2023 Jan 30. |
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| ID | Term |
|---|---|
| D009043 | Motor Activity |
| ID | Term |
|---|---|
| D001519 | Behavior |
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| ID | Term |
|---|---|
| D015444 | Exercise |
| ID | Term |
|---|---|
| D009043 | Motor Activity |
| D009068 | Movement |
| D009142 | Musculoskeletal Physiological Phenomena |
| D055687 | Musculoskeletal and Neural Physiological Phenomena |
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By using Enzyme-Linked Immunosorbent Assay (ELISA) the concentration of cytokines in the blood will be measured.
| At the beginning and at the end of the study at 4 weeks |
| Gene expression | By using real time reverse transcriptase polymerase chain reaction (RT-PCR) the concentration of messenger ribonucleic acid (mRNA) in cartilage tissue will be measured. | End of study at 4 weeks |
| Strength | By calculating the total volume of weight lifted (kg) the strength will be measured. | At the beginning and at the end of the study at 4 weeks |
| Body mass index (BMI) | BMI measured as the total body weight divided by the height squared (kg/m^2) | At the beginning of the study. |
| Age | The age measured in years | At the beginning of the study. |