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| Name | Class |
|---|---|
| Netherlands Organisation for Scientific Research | OTHER_GOV |
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This human intervention study will test if 12 weeks of supervised HIIT-based intervention improves skeletal muscle NOGD capacity in obese subjects.
19 overweight-obese (BMI => 27kg/m2), sedentary females and males aged 45-75yr will be enrolled in this study.
Participants will train 3 times/week under supervision during 12 weeks. Before, after and during this 12-week training period, there will be multiple metabolic measurements.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| HIIT | Experimental | This group of subjects will perform High Intensity Interval training 3x/week for 12 weeks |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| High-intenstiy interval training | Behavioral | High-intensity interval training is a training of 30 minutes involving 10 bouts of 1 minute high intensity cycling (80-90% of maximum heart rate) interspersed by 2 minutes rest. |
| Measure | Description | Time Frame |
|---|---|---|
| Non-oxidative glucose disposal | Measured during a 2-step hyperinsulinemic-euglycemic clamp combined with indirect calorimetry | 9 hours |
| Measure | Description | Time Frame |
|---|---|---|
| Skeletal muscle glycogen content | Assessed from skeletal muscle biopsies | 1 hour |
| Skeletal muscle insulin sensitivity | Measured during a 2-step hyperinsulinemic-euglycemic clamp |
| Measure | Description | Time Frame |
|---|---|---|
| Effect of consumption of insulinogenic, CHO-rich drink post-exercise | Investigate whether the consumption of a insulinogenic, CHO-rich drink post-training prevention adverse effects related to blood glucose fluctuation while maintaining the effects of 12 weeks of HIIT on NOGD. | 12 weeks |
Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Matthijs Hesselink, Prof. PhD. | Maastricht University | Principal Investigator |
| Vera Schrauwen-Hinderling, PhD | Maastricht University | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University Maastricht | Maastricht | Limburg | 6229ER | Netherlands |
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| ID | Term |
|---|---|
| D050177 | Overweight |
| D009765 | Obesity |
| ID | Term |
|---|---|
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
| D001835 | Body Weight |
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1 group of participants that performs 12 weeks of HIIT with metabolic measurements before and after this training period
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| 9 hours |
| 24 hour glycaemic profile | continuous glucose monitor | 48 hours |
| Skeletal muscle mitochondrial function assessed by Magnetic Resonance Spectroscopy Scan using 31P-MRS methodology, based on the phosphocreatine (PCr) recovery kinetics after exercise. | The participant is positioned in the scanner with a home-built exercise device to perform consecutive knee-extensions, in which the participant has to lift a weight whilst laying in the scanner (50-60% max leg capacity of the subjects) for 5 minutes. Scout images of the upper leg are acquired and fine-tuned shimming is applied. Before, during and after exercise, spectra are acquired every 4 seconds. The restoration of PCr is driven almost purely by oxidative metabolism, the time to restore the normal amount reflects mitochondrial function (a faster restoration time means better mitochondrial function). | 1 hour |
| Metabolic Flexibility during exercise assessed by indirect calorimetry. | Participants will undergo a standardized cycling test at low, moderate and high intensity (30%-50%-70%) during 10 minutes each. Throughout the test, indirect calorimetry will be performed in order to measure changes in substrate oxidation during exercise. | 30 minutes |
| Ectopic Fat accumulation in the liver assessed by magnetic resonance spectroscopy. | Participants will undergo a magnetic resonance spectroscopy scan from which hepatic fat content will be quantified. In the 1H-MRS spectra, the water signal, that is dominating the proton spectra, will be suppressed using frequency-selective pre-pulse and the spectra will be fitted to quantify the lipid peak. A separate spectrum will be measured without water suppression to quantify the unsuppressed water signal. The CH2/Water ratio will be used as parameter of intrahepatic lipid content. | 1 hour |
| Maximal Acetylcarnitine formation in the upper-leg at rest and after exercise assessed by Magnetic Resonance Spectroscopy Scan | Magnetic resonance imaging (MRI) will be used to guide the spectroscopy measurements and fine shimming will be performed to optimize the magnetic field homogeneity within the region of interest. A volume of interest will be selected within the m. vastus lateralis from the MRI images and the 1H-MRS spectra will be acquired from this region of interest. The intensity of the creatine signal will be used as internal reference. | 1 hour |
| D012816 |
| Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |