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Sickle cell patients have a high prevalence of alloimmunization. This high rate of alloimmunization can be partially explained by the existence of an antigenic difference between the predominantly Caucasian donor population and the sickle cell patients of African origin. Genetic and environmental risk factors have also been described.
The main risk factors that have been shown in retrospective or cross-sectional studies are some HLA alleles, the age of the patient, the number of leukocyte-depleted erythrocyte concentrates (CED) transfused, the number of transfusion episodes, the age of the CEDs, the existence of an inflammatory event at the time of transfusion and the presence of anti-erythrocyte autoantibodies.There is also evidence of an impaired TH response but the underlying immunological mechanism is not fully understood.
The aim of this study is to study the prevalence and the risk factors for anti-erythrocyte alloimmunization and to try to understand the immunological mechanisms.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Experimental group | Experimental | Allo-immunization detected (positive response for irregular antibodies 2 to 4 weeks after a blood transfusion) |
|
| Control group | Other | Allo-immunization not detected |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Blood sampling | Procedure | Extra blood sampling at the time of a blood transfusion in order to perform the laboratory analysis |
|
| Measure | Description | Time Frame |
|---|---|---|
| Irregular antibodies | Presence/abscence of irregular antibodies | 1 hour before blood transfusion |
| Irregular antibodies | Presence/abscence of irregular antibodies | Between 2 to 4 weeks after blood transfusion |
| C-reactive protein (CRP) | CRP dosage | 1 hour before blood transfusion |
| Cytokine | Cytokine dosage | 1 hour before blood transfusion |
| Cytokine | Cytokine dosage | Between 2 to 4 weeks after blood transfusion |
| Heme oxygenase | Heme oxygenase dosage | 1 hour before blood transfusion |
| Heme oxygenase | Heme oxygenase dosage | Between 2 to 4 weeks after blood transfusion |
| Lymphocyte typing | Lymphocyte typing | 1 hour before blood transfusion |
| Lymphocyte typing | Lymphocyte typing |
| Measure | Description | Time Frame |
|---|---|---|
| Sex | Sex | 1 hour before blood transfusion |
| Chronic or acute blood transfusion | Blood transfusions planned at regular intervals of time (chronic transfusions) or performed in reaction to a medical issue (acute transfusion). |
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Inclusion Criteria:
Sickle cell disease patients treated within the CHU Brugmann or Queen Fabiola Children's Hospital
Exclusion Criteria:
None
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| Name | Affiliation | Role |
|---|---|---|
| Marie Deleers, Ph Biol | CHU Brugmann | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| CHU Brugmann | Brussels | 1020 | Belgium | |||
| HUDERF |
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| ID | Term |
|---|---|
| D000755 | Anemia, Sickle Cell |
| ID | Term |
|---|---|
| D000745 | Anemia, Hemolytic, Congenital |
| D000743 | Anemia, Hemolytic |
| D000740 | Anemia |
| D006402 | Hematologic Diseases |
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| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
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| Between 2 to 4 weeks after blood transfusion |
| 1 hour before blood transfusion |
| Blood transfusion indication | Medical reason explaining the necessity of a blood transfusion | 1 hour before blood transfusion |
| Blood donor ethnicity | Blood donor ethnicity | 1 hour before blood transfusion |
| Brussels |
| 1020 |
| Belgium |
| D006425 |
| Hemic and Lymphatic Diseases |
| D006453 | Hemoglobinopathies |
| D030342 | Genetic Diseases, Inborn |
| D009358 | Congenital, Hereditary, and Neonatal Diseases and Abnormalities |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |