Estradiol, Progesterone and Endometrial Thickness in Frozen Euploid Embryo Transfer

Estradiol, Progesterone and Endometrial Thickness in Frozen Euploid Embryo Transfer

The development and improvement in Preimplantation genetic diagnosis/screening (PGS) in association with the efficacy and security of vitrification and trophectoderm biopsy, more efficient stimulation schemes and laboratory improvements in culture and embryo selection have contributed to high success of the assisted reproductive technologies(ART). Transferring an euploid embryo avoids one of the main reasons of miscarriage and implantation failure ( Rubio et al, 22016) and overcomes one of the most important confounding factors. Usually, the transfer of euploid embryos is performed under standard hormone replacement therapy (HRT) and are referred to as FET-PGS cycles( Frozen embryo transfer). The study of FET-PGS cycles with the transfer of a single euploid blastocyst is the best model for evaluation of the impact of the endometrial preparation . Among factors influencing FET outcomes, patient's age, endometrial preparation, endometrium thickness, endometrial pattern, number and quality of embryos transferred , difficulty at ET, recently P4 have received more attention .

The importance of Progesterone (P4) in embryo implantation has been widely studied. P4 plays an important role in endometrial gland formation, embryonic implantation and pregnancy maintenance, both in natural and artificial cycles.

Inclusion Criteria:

• Frozen embryo transfer (FET) of at least one single euploid embryo.
• Estradiol > 75pg/ml.
• Progesterone > 4 ng/ml.
• Endometrial thickness between 6 mm and 18 mm.

Exclusion Criteria:

• Patients with FET of no diagnostic or mosaic embryos, or known uterine abnormality were excluded.
• Natura cycle protocol.