randomized clinical trial which Evaluate thr Post-operative Pain Following Platelet Rich Plasma (PRP) Revascularization versus Conventional Endodontic Treatment of Non-vital Mature Mandibular Molars with Chronic Peri-apical Periodontitis

it will be done in 2 visits where in the firist visit, i will finish cleaning and shaping of the canals and inject double antibiotic paste as intracanal medication.

in the second visit, removal of intracanal medication then obturaton of the canals in the control group. while in the intervention group,over instrumentation of the canals beyond the working length using k-file size 20-35 to establish bleeding in the canals.then blood sample is collected for PRP preparation then injected into the canal.the patient will record post operative pain 6,12,24,48 hours then 3,4,5 days later.then they will do CBCT at 6, 12 months after the treatment to assess peri-apical healing

Inclusion Criteria:

• Medically free patients, between 18-40 years
• Patients with necrotic pulp in mandibular molar permanent teeth with closed apex

Exclusion Criteria:

• Illiterate patient

• Patients with systemic diseases

• Pregnant women

• Patients that have allergy to ciprofloxacin or metronidazole

• Teeth that:

  • Have vertical root fracture, coronal perforation, and calcification.
  • re-treatment cases
  • Non restorable

• Patients that have T.M.J. disorder