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10 patients inclued instead of 50
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This pilot study aims to study intestinal bacterial and fungal translocation and the evolution of the intestinal microbiota in patients over the course of their medical surveillance to search for a link between dysbiosis and bacterial/fungal translocation, but also to better understand the elements involved in febrile episodes in these patients (lack of detection of blood microorganisms, translocation of constituent elements of these microorganisms, etc.). We hypothesize that the composition of the intestinal microbiota as well as the phenomenon of intestinal microbial translocation will have an influence on the occurrence of fever and/or bacteremia in neutropenic patients hospitalized in pediatric onco-hematology.
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Evaluation of microbial translocation and microbiota | Diagnostic Test | Blood test from catheter already in place to determine microbial translocation and stool sample taken for microbiota analysis |
| Measure | Description | Time Frame |
|---|---|---|
| Evaluate the effect of presence of bacterial and fungal translocation on occurrence of episodes of febrile neutropenia in pediatric onco-hematology patients. | Presence or absence of microbial translocation; presence = ≥ 10 copies of rDNA16S/µL and/or ≥ 1 copies rDNA 18S/µL and ≥10 ng/mL LBP, ≥10 ng/mL of sCD14 and ≥5 ng/mL of zonulin. | Between day 7-15 |
| Measure | Description | Time Frame |
|---|---|---|
| Evaluate prognostic quality of fungal and bacterial translocation markers in occurrence of episodes of febrile neutropenia of unknown origin | Between day 7-15 | |
| Evaluate prognostic quality of fungal and bacterial translocation markers in occurrence of episodes of febrile neutropenia in bacteremic patients |
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Inclusion Criteria:
Exclusion Criteria:
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Patients presenting to the CHU Nîmes onco-pediatric service with febrile neutropenia
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| Name | Affiliation | Role |
|---|---|---|
| Jean-Philippe Lavigne | CHU Nimes | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| UFR de Pharmacie Laboratoire de Parasitologie et Mycologie MĂ©dicale | Montpellier | 34093 | France | |||
| CHU de Montpellier |
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Blood samples and stool samples
| Between day 7-15 |
| Compare bacterial and fungal translocation kinetics in febrile neutropenic patients of unknown origin versus bacteremic patients | Between day 7-15 |
| Compare direct (16S rDNA, 18S rDNA) versus indirect (LBP, sCD14 and plasma zonulin) measures of translocation and association with bioclinical characteristics of the population | Between day 7-15 |
| Describe the kinetics of markers and intestinal microbial phylogenetic compositions according to the bioclinical characteristics of the population | Diversity defined by number of different species and number of different taxonomic groups | Between day 7-15 |
| Creation of biobank | All blood and stool samples taken from patients who develop neutropenia. Patients who do not develop neutropenia will have their samples collected at inclusion destroyed | end of study day 30 |
| Montpellier |
| 342995 |
| France |
| CHU Nimes | Nîmes | 30029 | France |
| ID | Term |
|---|---|
| D064147 | Febrile Neutropenia |
| ID | Term |
|---|---|
| D009503 | Neutropenia |
| D000380 | Agranulocytosis |
| D007970 | Leukopenia |
| D000095542 | Cytopenia |
| D006402 | Hematologic Diseases |
| D006425 | Hemic and Lymphatic Diseases |
| D007960 | Leukocyte Disorders |
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| ID | Term |
|---|---|
| D064307 | Microbiota |
| ID | Term |
|---|---|
| D008827 | Microbiological Phenomena |
| D058448 | Biota |
| D044822 | Biodiversity |
| D017753 | Ecosystem |
| D004777 | Environment |
| D055669 | Ecological and Environmental Phenomena |
| D001686 | Biological Phenomena |
| D004778 | Environment and Public Health |
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