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| Name | Class |
|---|---|
| Arla Foods | INDUSTRY |
| Innovation foundation | UNKNOWN |
| Mejeribrugets ForskningsFond | OTHER |
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The primary aim of the trial is to determine the optimal dose of protein per meal in relation to enhancing muscle protein synthesis and thereby in perspective counteract muscle loss during weight loss. In addition, we aim to determine whether the response to protein intake is reduced in women on hypocaloric diet compared to in women on an energy-balanced diet.
Aim of this project is to determine the optimal protein dose (high quality milk protein) per meal in relation to enhancing muscle protein synthesis and thereby counteract muscle loss during weight loss.
The hypothesis is that the optimum dose of protein for women on a hypocaloric diet will be greater than when women eat an energy sufficient diet, and also higher than the optimal dose previously determined in weight stable men.
Methods: Forty women (~ 50 years) will be randomized to four groups. Following a five day hypocaloric diet (three groups) or an energy balanced diet (one group). The women will report to the laboratory to ingest one of three different protein doses. The muscle protein response to the different doses of protein will be determined by the muscle protein fractional synthetic rates using tracer kinetics.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| 15g protein hypocaloric diet | Experimental | Following a 5-day hypocaloric diet the subjects (n=10) will ingest 15g of protein and their mixed muscle protein synthesis response will be monitored using tracer kinetics. |
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| 35g protein hypocaloric diet | Experimental | Following a 5-day hypocaloric diet the subjects (n=10) will ingest 35g of protein and their mixed muscle protein synthesis response will be monitored using tracer kinetics. |
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| 60g protein hypocaloric diet | Experimental | Following a 5-day hypocaloric diet the subjects (n=10) will ingest 60g of protein and their mixed muscle protein synthesis response will be monitored using tracer kinetics. |
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| 35g protein energy balanced diet | Experimental | Following a 5-day energy balanced diet the subjects (n=10) will ingest 35g of protein and their mixed muscle protein synthesis response will be monitored using tracer kinetics. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| 15 g protein hypocaloric | Other | Following a 5 day hypocaloric diet one group of 10 women will ingest 15 g of protein |
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| Measure | Description | Time Frame |
|---|---|---|
| Fractional synthetic rate | Mixed muscle protein synthetic rate determined by tracer kinetics | 3 hours after ingestion of protein |
| Measure | Description | Time Frame |
|---|---|---|
| Muscle signalling | Markers for myofibrillar protein turnover measured by RT-PCR and western blotting | 3 hours after ingestion of protein. |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Aarhus University, Department for Public Health, Section for Sport Science | Aarhus | 8000 | Denmark |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 37598750 | Derived | Larsen MS, Witard OC, Holm L, Scaife P, Hansen R, Smith K, Tipton KD, Mose M, Bengtsen MB, Lauritsen KM, Mikkelsen UR, Hansen M. Dose-Response of Myofibrillar Protein Synthesis To Ingested Whey Protein During Energy Restriction in Overweight Postmenopausal Women: A Randomized, Controlled Trial. J Nutr. 2023 Nov;153(11):3173-3184. doi: 10.1016/j.tjnut.2023.08.011. Epub 2023 Aug 19. |
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: Forty women (~ 50 years) will be randomized to four groups. Following a five day hypocaloric diet (three groups) or an energy balanced diet (one group). The women will report to the laboratory to ingest one of three different protein doses.
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The protein doses provided will be masked. As all participants will receive the same amount and the drinks will taste similarly.
| 35 g protein hypocaloric | Other | Following a 5 day hypocaloric diet one group of 10 women will ingest 35 g of protein |
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| 60 g protein hypocaloric | Other | Following a 5 day hypocaloric diet one group of 10 women will ingest 60 g of protein |
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| 35 g protein energy balance | Other | Following a 5 day energy balanced diet one group of 10 women will ingest 35 g of protein |
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