Genotype, Metabotype and Diet in a Cohort of Children and Adolescents From the Southeast of Spain.

Association Between Genotype, Polyphenol Gut Microbiota Metabotypes, and Diet in a Cohort of Children and Adolescents From the Southeast of Spain.

The objective of this study is to investigate the possible association between 60 SNPs related to obesity, metabolism and cardiovascular disease with BMI, intestinal microbiota and adherence to the Mediterranean diet in a cohort of children and adolescents from the southeast of Spain.

The objective of this study is to investigate the possible association between 60 single nucleotide polymorphisms (SNPs) related to obesity, metabolism and cardiovascular disease with the body mass index (BMI), intestinal microbiota related to the metabolism of polyphehnos and adherence to the Mediterranean diet in a cohort of children and adolescents from the southeast of Spain.

The volunteers (n=423) with ages ranging from 5 to 18 years consumed for three days a daily serving of walnuts (30g) and provided a sample of urine (to determine mirobial-derived metabolites from walnut polyphenols) as well as a swab specimen from the inner cheek to provide DNA for further genotyping.

Inclusion Criteria:

• Healthy individuals aged 5 to 18 years.
• Informed consent signed by the parent / legal representative, and by volunteers who are already 18 years old.

Exclusion Criteria:

• Known or suspected allergy or intolerance to nuts or pomegranate.
• Intake of antibiotics 1 month before the start of the study.
• If the participant could perceive stress or psychological damage due to his participation.
• Any other circumstance different from previous ones (illness, etc.), according to which it is considered that the intervention in the study may involve some type of affectation.