Prevailing microorganisms causing neonatal sepsis in neonatal intensive care unit of Assiut University children Hospital Methods prospective study conducted in the neonatal intensive care unit in Assiut university children hospital.

Sepsis remains a leading cause of death in the intensive care units and in all age groups worldwide.sepsis was defined as the host's immune response(systemic inflammatory response syndrome) to injury and/or infectious stimuli in the presence of a known(or strongly suspected) infection .early onset neonatal sepsis occurs in utero or might become infected when exposed to potentially pathogenic bacteria,viruses,or fungi during passage through the birth canal.

Inclusion Criteria:

• both preterm and full term neonates
• both early onset and late onset neonatal sepsis

Exclusion Criteria:

• Genetic diseases immunocompromized neonates
• Neonates of diabetic mothers
• Neonates of HIV mothers

Neonates from age zero to 28 days