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The research project cannot be implemented because the project has not received funding subsidies
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In this project, PBMCs will be isolated from HDM allergic and non allergic patient and then stimulated with HDM crude extract. The expression of inflammasome and the correlation of inflammasome activation and steroid resistance will be investigated.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Steroid resistance in HDM allergic patients | Experimental |
| |
| Steroid resistance in non HDM allergic subjects | Experimental |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| House dust mite | Biological | As a stimulant to induce inflammasome activation |
|
| Measure | Description | Time Frame |
|---|---|---|
| inflammasome activation by HDM | The expression of NLRP3 in PBMCs will be measured by western blot after HDM stimulation. | 6 hours |
| Steroid receptor phosphorylation by HDM | The expression of steroid receptor will be detected by western blot and real-time PCR after HDM stimulation. | 6 hours |
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Inclusion Criteria:
Exclusion Criteria:
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| ID | Term |
|---|---|
| D039741 | Antigens, Dermatophagoides |
| ID | Term |
|---|---|
| D000941 | Antigens |
| D001685 | Biological Factors |
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