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Recent data suggest that sperm cells carry an epigenetic message during spermatogenesis and that this message is crucial for the future development of the embryo. This epigenetic signature is notably represented by methylation of genes subjected to imprinting (GSI) and the methylation of transposable elements (TE). Data on the maintenance of the imprint and of the control of TE accompanying human gametogenesis in a context of adult germinal testicular cancers, seminomas, are extremely fragmentary for tumour tissues and inexistent for gametes.
The aim of this study is to determine whether patients with seminomas in comparison with fertile men carry a higher risk of presenting epigenetic alterations affecting their gametes.
This study is based on the use of an existing collection of biological samples. 90 samples will be selected and split into 3 groups:
After treatment of the samples (thawing, cell sorting and removal of cryoprotectants), they will be analysed.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| testicular tumours |
| ||
| fertile |
| ||
| infertile |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Extraction genomic DNA | Other |
| ||
| Analysis of methylation profiles of numerous GSI and TE |
| Measure | Description | Time Frame |
|---|---|---|
| Analysis of methylation profiles in paternal genes subjected to imprinting (GSI) | at baseline |
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Inclusion Criteria:
Exclusion Criteria:
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seminomas
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| CHU Dijon Bourgogne | Dijon | 21079 | France |
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| Other |
|
| Analysis of methylation for the whole genome (sub-population of 15 samples from group 1) | Other |
|
| Method of protein detection by immunostaining and flow cytometry | Other |
|
| ID | Term |
|---|---|
| D018239 | Seminoma |
| ID | Term |
|---|---|
| D018237 | Germinoma |
| D009373 | Neoplasms, Germ Cell and Embryonal |
| D009370 | Neoplasms by Histologic Type |
| D009369 | Neoplasms |
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| ID | Term |
|---|---|
| D005434 | Flow Cytometry |
| ID | Term |
|---|---|
| D002469 | Cell Separation |
| D003584 | Cytological Techniques |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
| D003592 | Cytophotometry |
| D005470 | Fluorometry |
| D008163 | Luminescent Measurements |
| D010783 | Photometry |
| D002623 | Chemistry Techniques, Analytical |
| D008919 | Investigative Techniques |
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