Constipated patients often have mental problems such as depression and anxiety due to difficult defecation. Our previous studies have proved the efficacy of FMT treating constipation. Meanwhile it is believed that mental diseases are correlated to gut microbiota. This trial is based on the theory of the gut-brain-microbiota axis. Patients with constipation, depression and/or anxiety are performed FMT, laboratory, imaging and microbiota examinations, and clinical follow-up, to observe the clinical efficiency of FMT and the potential role of gut microbiome in these gut-brain disorders.

Inclusion Criteria:

• Patients who were diagnosed as functional constipation (FC) according to Rome Ⅳ criteria were enrolled.
• Age ≥ 18 years
• Body mass index of 18-25 kg/m2.
• HAMA ≥ 14 and/or HAMD (17 items) ≥ 17

Exclusion Criteria:

• History of sever mental disorders such as schizophrenia and bipolar disorder
• History of organic intestinal disorders
• History of gastrointestinal surgery
• Pregnant or breast-feeding women
• Infection with enteric pathogen
• Usage of probiotics, prebiotics, antibiotics within the last month
• Uncontrolled hepatic, renal, cardiovascular, and respiratory, or any diseases that in the opinion of the investigator, could affect intestinal transit and the intestinal microbiota.
• Patients who could not complete the follow-up