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The present study is an effort to investigate the hypothesis that Platelet Rich Fibrin/Biphasic Calcium Phosphate may play a role in inhibiting osteoclasts differentiation and in bone loss.
Study design
Fifty-five individuals from a suburban population were recruited. Out of them, 5 individuals refused to participate in the study. Finally, 50 participants were selected and divided into two groups, control group [Healthy individuals]: 25 Males/Females, aged 25 to 35 years and test group [Chronic periodontitis patients]: 25 Males/Females aged 30 to 45 years.
Demographic variables and periodontal parameters were measured in both the groups.The investigators explored the effect of Platelet rich fibrin (PRF)/Biphasic calcium phosphate (BCP) on differentiation and survival of osteoclasts obtained from peripheral blood of Chronic Periodontitis patients.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Test group | Experimental | Mononuclear cells isolated from the peripheral blood of 25 chronic periodontitis patients were interveened with 1ml/well preparation of Platelet rich fibrin(PRF), Biphasic calcium phosphate (BCP) and PRF and BCP combination in-vitro. |
|
| control group | Other | Mononuclear cells isolated from the peripheral blood of healthy individuals in-vitro. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| other : PRF | Other | mononuclear cells from the peripheral blood of chronic periodontitis patients were intervened with PRF, BCP and PRF/BCP combination |
|
| Measure | Description | Time Frame |
|---|---|---|
| probing pocket depth | probing pocket dept was measured using Williams periodontal probe at base line | base line |
| Measure | Description | Time Frame |
|---|---|---|
| Protein expression on osteoclast cells was estimated with BCP, PRF alone and BCP/PRF combination. | Protein expression on cells was analyzed using Western blot in all the groups | 24 hours from baseline |
| The activity of caspase-3 in OCs. |
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Inclusion criteria: (Control group)
Inclusion Criteria: (Test group)
Exclusion Criteria: ( both the groups)
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| Name | Affiliation | Role |
|---|---|---|
| shila samuel, MS, PhD | VRR diagnostic institute chennai | Principal Investigator |
| jaideep mahendra, MDS,PhD | meenakshi ammal dental college chennai | Principal Investigator |
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| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 22624763 | Result | Wu CL, Lee SS, Tsai CH, Lu KH, Zhao JH, Chang YC. Platelet-rich fibrin increases cell attachment, proliferation and collagen-related protein expression of human osteoblasts. Aust Dent J. 2012 Jun;57(2):207-12. doi: 10.1111/j.1834-7819.2012.01686.x. | |
| 16848983 | Result | Colucci S, Mori G, Brunetti G, Coricciati M, Pignataro P, Oranger A, Cirulli N, Mastrangelo F, Grassi FR, Grano M. Interleukin-7 production by B lymphocytes affects the T cell-dependent osteoclast formation in an in vitro model derived from human periodontitis patients. Int J Immunopathol Pharmacol. 2005 Jul-Sep;18(3 Suppl):13-9. |
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| ID | Term |
|---|---|
| D010510 | Periodontal Diseases |
| D016301 | Alveolar Bone Loss |
| D001862 | Bone Resorption |
| ID | Term |
|---|---|
| D009059 | Mouth Diseases |
| D009057 | Stomatognathic Diseases |
| D001847 | Bone Diseases |
| D009140 | Musculoskeletal Diseases |
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Control group: (Healthy individuals) comprised of 25 participants with clinically healthy periodontium.
Test group: (Chronic periodontitis patients) comprised of 25 participants with signs of clinical inflammation and bone loss.
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|
The activity of caspase-3 was seen in OCs in all the groups
| 24 hours from base line |
| D055093 |
| Periodontal Atrophy |