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The aim of this study is to investigate the In Vitro and In Vivo effect of progesterone on immature myeloid cells (IMC), inflammation characteristics and maturation into dendritic cells (DC).
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Preterm labor placentas and progesterone | Active Comparator | IMC cells taken from preterm labor placentas after delivery will be cultured with progesterone and will be analyzed for inflammation characteristics and maturation into DC cells. |
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| Term labor placentas and progesterone | Active Comparator | IMC cells taken from term labor placentas after delivery will be cultured with progesterone and will be analyzed for inflammation characteristics and maturation into DC cells. |
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| Preterm labor placentas without progesterone | Active Comparator | IMC cells taken from preterm labor placentas after delivery will be cultured without progesterone and will be analyzed for inflammation characteristics and maturation into DC cells. |
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| Term labor placentas without progesterone | Active Comparator | IMC cells taken from term labor placentas after delivery will be cultured without progesterone and will be analyzed for inflammation characteristics and maturation into DC cells. |
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| Term placentas from progesterone treated pregnancies | Active Comparator |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Culture with progesterone | Biological | Progesterone, a steroid hormone will be cultured with placental IMC cells or peripheral blood CD14+ monocyte cells |
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| Measure | Description | Time Frame |
|---|---|---|
| Inflammation characteristic effects of progesterone on preterm and term placentas | Differences observed in cytokine profiles of IMC cells from term and preterm placentas cultured with and without progesterone | One year |
| Maturation effects of progesterone on preterm and term placentas | Differences observed in maturation of IMC cells into DC cells in term and preterm placentas | One year |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Rinat Gabbay-Benziv, MD | Hillel Yaffe Medical Center | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Hillel Yaffe Medical Center | Hadera | 38100 | Israel |
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| ID | Term |
|---|---|
| D007752 | Obstetric Labor, Premature |
| ID | Term |
|---|---|
| D007744 | Obstetric Labor Complications |
| D011248 | Pregnancy Complications |
| D005261 | Female Urogenital Diseases and Pregnancy Complications |
| D000091642 | Urogenital Diseases |
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| ID | Term |
|---|---|
| D046508 | Culture Techniques |
| D011374 | Progesterone |
| D036341 | Intercellular Signaling Peptides and Proteins |
| ID | Term |
|---|---|
| D066298 | In Vitro Techniques |
| D008919 | Investigative Techniques |
| D011282 | Pregnenediones |
| D011283 | Pregnenes |
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IMC cells taken from term labor placentas from women that were treated with progesterone during pregnancy will be analyzed for inflammation characteristics and maturation into DC cells. |
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| Preterm placentas from progesterone treated pregnancies | Active Comparator | IMC cells taken from preterm labor placentas from women that were treated with progesterone during pregnancy will be analyzed for inflammation characteristics and maturation into DC cells. |
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| Term placentas not treated with progesterone in pregnancy | Active Comparator | IMC cells taken from term placentas from women who were not treated with progesterone during pregnancy will be analyzed for inflammation characteristics and maturation into DC cells. |
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| Preterm placentas not treated with progesterone in pregnancy | Active Comparator | IMC cells taken from preterm placentas from women who were not treated with progesterone during pregnancy will be analyzed for inflammation characteristics and maturation into DC cells. |
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| Term postpartum blood samples with progesterone | Active Comparator | CD-14+ monocyte cells taken from women delivering at term will be cultured with progesterone and will be analyzed for inflammation characteristics and maturation into DC cells. |
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| Preterm postpartum blood samples with progesterone | Active Comparator | CD-14+ monocyte cells taken from women delivering preterm will be cultured with progesterone and will be analyzed for inflammation characteristics and maturation into DC cells. |
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| Term postpartum blood samples without progesterone | Active Comparator | CD-14+ monocyte cells taken from women delivering at term will be cultured without progesterone and will be analyzed for inflammation characteristics and maturation into DC cells. |
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| Preterm postpartum blood samples without progesterone | Active Comparator | CD-14+ monocyte cells taken from women delivering preterm will be cultured without progesterone and will be analyzed for inflammation characteristics and maturation into DC cells. |
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| Culture without progesterone | Biological | Culture of placental IMC cells or peripheral blood CD14+ monocyte cells without progesterone |
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| Culture with growth factors | Biological | Culture of placental IMC cells with growth hormones only and analysis |
|
| D011278 |
| Pregnanes |
| D013256 | Steroids |
| D000072473 | Fused-Ring Compounds |
| D011083 | Polycyclic Compounds |
| D003339 | Corpus Luteum Hormones |
| D042341 | Gonadal Hormones |
| D006728 | Hormones |
| D006730 | Hormones, Hormone Substitutes, and Hormone Antagonists |
| D045167 | Progesterone Congeners |
| D012739 | Gonadal Steroid Hormones |
| D010455 | Peptides |
| D000602 | Amino Acids, Peptides, and Proteins |
| D011506 | Proteins |
| D001685 | Biological Factors |