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| Name | Class |
|---|---|
| National University of Singapore | OTHER |
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Hippo-YAP pathway is a newly discovered pathway controlling the growth, and plays important roles in the regulation of stem cell function and tissue regeneration. Previously we found that a higher expression of YAP in NP, and LPS was able to activate the hippo-YAP pathway in NESCs. Thus, we hypothesize that the aberrant proliferation and differentiation of NESCs could be regulated via the hippo-YAP pathway.
Studies have shown that the development of nasal polyps (NP) are closely related to the epithelial remodeling.The investigators have shown that nasal epithelial stem cells(NESCs) derived from NP had reduced growth and proliferation dynamics in vitro culture,and differentiated into ciliated cells with impaired cilia architecture and ciliogenesis.Further studies demonstrated that NESCs were involved in the aberrant damage repair process of the epithelial remodeling in NP.Hippo-YAP pathway is a newly discovered pathway controlling the growth,and plays important roles in the regulation of stem cell function and tissue regeneration.Previously the investigators found that a higher expression of YAP in NP,and LPS was able to activate the hippo-YAP pathway in NESCs.Thus,the investigators hypothesize that the aberrant proliferation and differentiation of NESCs could be regulated via the hippo-YAP pathway,which may result in the epithelial remodeling.In this study,the investigators intend to investigate the mechanism of the hippo-YAP pathway in regulating aberrant proliferation and differentiation of NESCs and explore its upstream signaling and downstream target genes,using the NESCs gas-liquid interface culture system,and a silencing and overexpression of the hippo-YAP pathway component strategy.The investigators aim to unravel the effect and mechanism of the hippo-YAP pathway in the epithelial remodeling of NP,providing new targets and strategy for the improvement or reversal of NP epithelial remodeling.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| nasal polyps | Experimental |
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| inferior turbinate | Placebo Comparator | Compared with nasal polyps,explore the levels of LPS and Th2/17 cytokines in nasal polyps,and the relation between cytokines and the activation of hippo-YAP pathway through Immunohistochemistry and Immunofluorescence（P63、Ki67、YAP、LATS1/2 and MST1/2），as well as Western Blot and the Flow cytometry. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| functional endonasal sinus surgery | Procedure | collect nasal polyps from patients |
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| Measure | Description | Time Frame |
|---|---|---|
| location of YAP in tissues | through Immunohistochemistry | through study completion, an average of 2 years |
| protein expression level of YAP in tissues | through western blot | through study completion, an average of 2 years |
| Measure | Description | Time Frame |
|---|---|---|
| expression level of Th1 cytokines in the tissues | through Flow cytometry | through study completion, an average of 2 years |
| expression level of Th17 cytokines in the tissues | through Flow cytometry |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| huiyi DENG, MD | Contact | 15521127735 | deng.huiyi@foxmail.com | |
| qintai YANG, PhD | Contact | 13724859848 | yang.qt@126.com |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| ThirdSunYatSen | Recruiting | Guangzhou | Guangdong | 510630 | China |
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| ID | Term |
|---|---|
| D009298 | Nasal Polyps |
| ID | Term |
|---|---|
| D009668 | Nose Diseases |
| D012140 | Respiratory Tract Diseases |
| D010038 | Otorhinolaryngologic Diseases |
| D011127 | Polyps |
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| nasal septal construction | Procedure | collect inferior turbinates from patients as control arm |
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| through study completion, an average of 2 years |
| activation of hippo-YAP in NSECs on protein aspect | Western Blot | through study completion, an average of 2 years |
| activation of hippo-YAP in NSECs | Flow cytometry | through study completion, an average of 2 years |
| activation of hippo-YAP in NSECs on gene aspect | quantitive real time PCR | through study completion, an average of 2 years |
| D020763 |
| Pathological Conditions, Anatomical |
| D013568 | Pathological Conditions, Signs and Symptoms |