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To explore the epigenetic mechanism of postoperative analgesia and side effect induced by μ-opioid Receptor Agonists presented with sufentanil among general population.
Patients were interviewed the day before surgery, and taught about the use of PCA pump and VAS. Pressure pain threshold(PPT) and pressure pain tolerance(PTO) were collected before surgery.
Dexmedetomidine1μg/Kg，sufentanil 0.5μg/Kg， propofol 2mg/Kg and rocuronium 0.6mg/Kg were given intravenously for induction. Anesthesia was maintained with inhalation of 1% sevoflurane and infusion of remifentanil (0.2-0.4ug/kg/min) and propofol (6-10mg/kg/h). Aterial blood pressure(ABP)，central venous pressure(CVP)，SPO2， HR，ETCO2，T and Narcotrend were monitored. Before incision, parecoxib 40mg was given intravenously, and PCA with sufentanil 1ug/ml was started immediately after surgery, suing a controlled infusion pump. The pump was programmed to use a loading dose of 2ml, background infusion at 2m/h, PCA dose of 1ml, lockout time of 10min, and maximal dose of 12ml with 1 h period.
VAS(static), VAS(dynamic), Ramssay, HR, NBP, SpO2, PCA pressing frequency, PCA comsumption were recorded 6h, 12h, 24h, 48h after surgery. Side effects such as nausea, vomiting, respiratory depression; pruritus; abdominal distention; urinary retention and dizziness were also recorded, and corresponding treatment were given.
EDTA anti-coagulated blood was collected from a central venous catheter during the operation. Genomic DNA was extracted from the blood samples, and characteristics and degree of DNA methylation of the gene OPRM1 were analysed.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| patients undergoing pancreatectomy | Experimental | patients undergoing pancreatectomy received dexmedetomidine 1μg/Kg，sufentanil 0.5μg/Kg， propofol 2mg/Kg，rocuronium 0.6mg/Kg for induction.And received sevoflurane(1-2%), remifentanil(0.1-0.2ug/kg/min) and propofol(0.3-0.6mg/kg/h) for maintenance. Parecoxib 40mg was given single intravenously before incision. And PCA with sufentanil 1ug/ml was started immediately after surgery. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Sufentanil | Drug | Patients received intravenous sufentanil 0.5μg/kg for induction,and received PCA after surgery with sufentanil 1ug/ml using a controlled infusion pump, which was programmed to use a loading dose of 2ml, background infusion at 2m/h, PCA dose of 1ml, lockout time of 10min, and maximal dose of 12ml within 1 hour period. |
| Measure | Description | Time Frame |
|---|---|---|
| Quantification of methylation in CpG islands located in gene OPRM1 | EDTA anti-coagulated blood was collected before induction. Genomic DNA was extracted according to the manufacturer's protocol, and CpG islands in the OPRM1 gene region were identified by CpG Island Explorer 2.0 software. Quantification of DNA methylation of all CpG sites from position +528 to +1649 in CpG islands was analysed. | 3 months |
| Measure | Description | Time Frame |
|---|---|---|
| Pressure pain threshold (PPT) and pressure pain tolerance (PTO) | Patients' Pressure pain threshold(PPT) and pressure pain tolerance(PTO) in Kg/cm2 were measured before surgery using electronic pressure algometer (YISIDA-DS2, Hongkong, China). | 1 day |
| Sex |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Ai Ling | Contact | +8613667135571 | aileen911@126.com | |
| Xu Hui | Contact | +8613971001596 |
| Name | Affiliation | Role |
|---|---|---|
| Xu Hui | Department of Anaesthesiology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology | Study Chair |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Department of Anaesthesiology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology | Recruiting | Wuhan | Hubei | 430030 | China |
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| ID | Term |
|---|---|
| D017409 | Sufentanil |
| D020927 | Dexmedetomidine |
| D015742 | Propofol |
| D000077123 | Rocuronium |
| D000077149 | Sevoflurane |
| D000077208 | Remifentanil |
| C409945 | parecoxib |
| ID | Term |
|---|---|
| D005283 | Fentanyl |
| D010880 | Piperidines |
| D006573 | Heterocyclic Compounds, 1-Ring |
| D006571 | Heterocyclic Compounds |
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| dexmedetomidine | Drug | Patients received intravenous dexmedetomidine 1μg/Kg for induction. |
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| propofol | Drug | Patients received intravenous propofol 2mg/Kg for induction, and received intavenous pump of propofol (0.3-0.6mg/kg/h) for maintenance. |
|
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| Rocuronium | Drug | Patients received intravenous rocuronium 0.6mg/Kg for induction. |
|
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| sevoflurane | Drug | Patients received inhalation of sevoflurane (1-2%) for maintenance. |
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| remifentanil | Drug | Patients received intavenous pump of remifentanil (0.1-0.2ug/kg/min) for maintenance. |
|
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| Parecoxib | Drug | Parecoxib 40mg was given single intravenously before incision. |
|
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Patients' sex (male or female) was recorded before surgery. |
| 1 day |
| Age | Patients' age in years was recorded before surgery. | 1 day |
| Weight | Patients' weight in kilograms was recorded before surgery. | 1 day |
| Height | Patients' height in meters was recorded before surgery. | 1 day |
| History of smoking | Patients' history of smoking (yes or no) was recorded before surgery. | 1 day |
| VAS (static) and VAS (dynamic) | Patients' VAS(static) and VAS(dynamic) score (range 0-10) were recorded at time point of 6hours, 12hours, 24hours, 48hours after surgery. | 6hours, 12hours, 24hours, 48hours after surgery. |
| Ramssay | Patients' Ramssay score (range 1-6) was recorded at time point of 6hours, 12hours, 24hours, 48hours after surgery. | 6hours, 12hours, 24hours, 48hours after surgery. |
| Heart rate (HR) | Patients' HR in bpm was recorded at time point of 6hours, 12hours, 24hours, 48hours after surgery. | 6hours, 12hours, 24hours, 48hours after surgery |
| Blood pressure (BP) | Patients' BP in mmHg was recorded at time point of 6hours, 12hours, 24hours, 48hours after surgery. | 6hours, 12hours, 24hours, 48hours after surgery. |
| Pulse oxygen saturation (SpO2) | Patients' SpO2 in percent was recorded at time point of 6hours, 12hours, 24hours, 48hours after surgery. | 6hours, 12hours, 24hours, 48hours after surgery. |
| PCA pressing frequency | Patients' PCA pressing frequency in time was recorded at time point of 6hours, 12hours, 24hours, 48hours after surgery. | 6hours, 12hours, 24hours, 48hours after surgery |
| PCA consumption | Patients' PCA consumption in ml was recorded at time point of 6hours, 12hours, 24hours, 48hours after surgery. | 6hours, 12hours, 24hours, 48hours after surgery |
| Side effects | Side effects such as nausea, vomiting, respiratory depression, pruritus, abdominal distention, urinary retention and dizziness (yes or no) were recorded at time point of 6hours, 12hours, 24hours, 48hours after surgery, and corresponding treatment were given. | 6hours, 12hours, 24hours, 48hours after surgery |
| D007093 |
| Imidazoles |
| D001393 | Azoles |
| D010636 | Phenols |
| D001555 | Benzene Derivatives |
| D006841 | Hydrocarbons, Aromatic |
| D006844 | Hydrocarbons, Cyclic |
| D006838 | Hydrocarbons |
| D009930 | Organic Chemicals |
| D000732 | Androstanols |
| D000731 | Androstanes |
| D013256 | Steroids |
| D000072473 | Fused-Ring Compounds |
| D011083 | Polycyclic Compounds |
| D008738 | Methyl Ethers |
| D004987 | Ethers |
| D006845 | Hydrocarbons, Fluorinated |
| D006846 | Hydrocarbons, Halogenated |
| D011422 | Propionates |
| D000144 | Acids, Acyclic |
| D002264 | Carboxylic Acids |