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The purpose of this study is to investigate the potential desensitising effects of repeated applications of capsaicin, trans-cinnamaldehyde and L-menthol on thermal, mechanical and chemical sensory stimulations.
Capsaicin, the compound responsible for the pungency of chili peppers, can induce desensitization to nociceptive (1) and pruriceptive (2) stimuli when topically applied. The purpose of this study is to determine whether repeated topical applications of trans-cinnamaldehyde and L-menthol result in decreased responses to various nociceptive and non-nociceptive thermal and mechanical stimuli and to compare potential alterations with those caused by repeated topical application of capsaicin. Furthermore, this study investigates the temporal profile of said alterations.
Capsaicin 8% patches, trans-cinnamaldehyde 10%, L-menthol 40% and inert vehicle patches will each be applied to 4 4x4 cm predefined areas on the skin of the volar forearms for 1 hour 4 times on 4 consecutive days. Before each application and the day after the last application, cold detection threshold (CDT), warmth detection threshold (WDT), cold pain threshold (CPT) and heat pain threshold (HPT) will be assessed using a Medoc Pathway (Medoc, Israel) equipped with a 3x3 cm contact thermode. On the last day, mechanical detection threshold (MDT) is assessed using a series of von Frey filaments and mechanical pain threshold (MPT) and mechanical pain sensitivity (MPS) is assessed using a series of standardized pinprick stimulators (Aalborg University).
Furthermore, full-field laser perfusion imaging (FLPI) will be used to assess the degree of neurogenic inflammation in each predefined area before and after each substance application and at the last session.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Capsaicin | Experimental | Capsacin (8%) patches applied topically 4 times for 1 hour to a 4x4 cm predefined area on the skin of the volar forearms. |
|
| Trans-cinnamaldehyde | Experimental | Trans-cinnamaldehyde (10%, dissolved in 90% ethanol) applied topically 4 times for 1 hour to a 4x4 cm predefined area on the skin of the volar forearms on a cotton ball placed in a plastic chamber to limit evaporation. |
|
| L-menthol | Experimental | L-menthol (40%, dissolved in 96% ethanol) applied topically 4 times for 1 hour to a 4x4 cm predefined area on the skin of the volar forearms on a cotton ball placed in a plastic chamber to limit evaporation. |
|
| Vehicle patch | Placebo Comparator | Inert vehicle patches applied topically 4 times for 1 hour to a 4x4 cm predefined area on the skin of the volar forearms. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Capsaicin | Drug | 4x4 cm patch of 8% topical capsaicin applied to the skin of the volar forearm for 1 hour 4 times on 4 consecutive days |
|
| Measure | Description | Time Frame |
|---|---|---|
| Cold Pain Threshold (CPT) | The pain threshold for cold stimulation assessed using a Medoc Pathway (Medoc, Israel) equipped with a 3x3 cm thermode. CPT is defined as the temperature at which cold induces pain and is expressed in °C. | Assessed at 5 visits on 5 consecutive days |
| Heat Pain Threshold (HPT) | The pain threshold for heat stimulation | Assessed at 5 visits on 5 consecutive days |
| Measure | Description | Time Frame |
|---|---|---|
| Neurogenic Inflammation Intensity | The average intensity of the neurogenic inflammation as assessed by full-field laser perfusion imaging (FLPI) (expressed in arbitrary units) | Assessed before and after application of substances at 5 visits on 5 consecutive days |
| Neurogenic Inflammation Area |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Lars Arendt-Nielsen, Professor | Contact | 0045 99408830 | lan@hst.aau.dk |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| SMI | Recruiting | Aalborg | 9000 | Denmark |
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| ID | Term |
|---|---|
| D010146 | Pain |
| D011537 | Pruritus |
| D009437 | Neuralgia |
| ID | Term |
|---|---|
| D009461 | Neurologic Manifestations |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D012871 | Skin Diseases |
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| ID | Term |
|---|---|
| D002211 | Capsaicin |
| C012843 | cinnamaldehyde |
| D008610 | Menthol |
| ID | Term |
|---|---|
| D053284 | Polyunsaturated Alkamides |
| D000577 | Amides |
| D009930 | Organic Chemicals |
| D000475 | Alkenes |
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| Trans-cinnamaldehyde | Drug | 1mL of 10% trans-cinnamaldehyde applied to the skin of the volar forearm for 1 hour 4 times on 4 consecutive days |
|
| L-Menthol | Drug | 1mL of 40% L-menthol applied to the skin of the volar forearm for 1 hour 4 times on 4 consecutive days |
|
| Vehicle patch | Drug | Inert vehicle patch applied to the skin of the volar forearm for 1 hour 4 times on 4 consecutive days |
|
The area of the neurogenic inflammation as assessed by full-field laser perfusion imaging (FLPI) (expressed in arbitrary units) |
| Assessed before and after application of substances at 5 visits on 5 consecutive days |
| Pain During Application of Capsaicin | Pain during the 60 minute application of topical capsaicin as rated on a visual analog scale (VAS) (0-10) | Assessed every 5 minutes during the 60 minute application of capsaicin at 4 visits on 4 consecutive days |
| Pain During Application of Trans-cinnamaldehyde | Pain during the 60 minute application of trans-cinnamaldehyde as rated on a visual analog scale (VAS) (0-10) | Assessed every 5 minutes during the 60 minute application of trans-cinnamaldehyde at 4 visits on 4 consecutive days |
| Pain During Application of L-menthol | Pain during the 60 minute application of L-menthol as rated on a visual analog scale (VAS) (0-10) | Assessed every 5 minutes during the 60 minute application of L-menthol at 4 visits on 4 consecutive days |
| Pain During Application of Inert Patch (Control) | Pain during the 60 minute application of the inert patch (control) as rated on a visual analog scale (VAS) (0-10) | Assessed every 5 minutes during the 60 minute application of the inert patch (control) at 4 visits on 4 consecutive days |
| Cold Detection Threshold (CDT) | The detection threshold for cold stimulation assessed using a Medoc Pathway (Medoc, Israel) equipped with a 3x3 cm thermode. CDT is defined as the temperature at which cold can be detected and is expressed in °C. | Assessed at 5 visits on 5 consecutive days |
| Warmth Detection Threshold (WDT) | The detection threshold for warmth stimulation assessed using a Medoc Pathway (Medoc, Israel) equipped with a 3x3 cm thermode. WDT is defined as the temperature at which warmth can be detected and is expressed in °C. | Assessed at 5 visits on 5 consecutive days |
| Mechanical Detection Threshold (MDT) | The detection threshold for mechanical stimulation is defined as the smallest force that can be felt and is assessed using a series of standardized von Frey filaments and using the "method of limits" employing a series of stimulations with ascending and descending force expressed in mN. | Assessed at day 5 (final visit) |
| Mechanical Pain Threshold (MPT) | The pain threshold for mechanical stimulation is defined as the smallest force that induces pain and is assessed using a series of standardized pinprick stimulators (Aalborg University) and using the "method of limits" employing a series of stimulations with ascending and descending forces expressed in mN. | Assessed at day 5 (final visit) |
| Mechanical Pain Sensitivity (MPS) | The sensitivity to mechanically induced pain induced by a series of 7 standardized pinprick stimulators (Aalborg University) as rated on a visual analog scale (VAS) ranging from 0-10 with 0 representing no pain and 10 representing the worst pain imaginable. | Assessed at day 5 (final visit) |
| Vibration Detection Threshold | The detection threshold for vibrational stimulation assessed using a Vibrameter (Somedic, Sweden). | Assessed at day 5 (final visit) |
| D017437 | Skin and Connective Tissue Diseases |
| D012877 | Skin Manifestations |
| D010523 | Peripheral Nervous System Diseases |
| D009468 | Neuromuscular Diseases |
| D009422 | Nervous System Diseases |
| D006839 |
| Hydrocarbons, Acyclic |
| D006838 | Hydrocarbons |
| D002396 | Catechols |
| D010636 | Phenols |
| D001555 | Benzene Derivatives |
| D006841 | Hydrocarbons, Aromatic |
| D006844 | Hydrocarbons, Cyclic |
| D012991 | Solanaceous Alkaloids |
| D000470 | Alkaloids |
| D006571 | Heterocyclic Compounds |
| D005229 | Fatty Acids, Monounsaturated |
| D005231 | Fatty Acids, Unsaturated |
| D005227 | Fatty Acids |
| D008055 | Lipids |
| D003511 | Cyclohexanols |
| D000441 | Hexanols |
| D005233 | Fatty Alcohols |
| D000438 | Alcohols |
| D000081005 | Cyclohexane Monoterpenes |
| D003510 | Cyclohexanes |
| D003516 | Cycloparaffins |
| D006840 | Hydrocarbons, Alicyclic |
| D039821 | Monoterpenes |
| D013729 | Terpenes |