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Analyze the effect of preincubation time of oocyte on the results of ICSI in a oocyte donation model.
There is an intense communication inside the follicular structure. Bidirectional information between the oocyte and the granulosa cells is needed for a normal follicular development and oocyte competence.
There are conflicting results on the effect of pre-incubation time in ICSI.
It is not clear the proper time to denudate the oocyte from the somatic cells prior to ICSI, and some authors describe a negative impact of the premature cummulus removal on the ICSI results.
The aim of this study is to investigate wheter a preincubation time between oocyte retireval and injection has any effect on the ICSI ( intracytoplasmatic sperm injection) results .
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Immediately oocyte denudation | Experimental | After the retrieval , sybling oocytes were assigned to one o more recipient and then ,the oocytes were randomized in two arms . The first arm is the immediately denudation and immediately ICSI of the oocytes, and in the second arm the oocyte are denuded and injected after 4 hours from the pick up . |
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| Delayed oocyte denudation | Experimental | After the retrieval , sybling oocytes were assigned to one o more recipient and then ,the oocytes were randomized in two arms . In this arm, the oocyte are denuded and injected after 4 hours from the pick up . |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| timing for oocyte denudation and Intracytoplasmatic Sperm injection | Procedure | Donated oocytes were randomly allocated to two groups concerning the time of cumulus corona removal. The first group: After oocyte retrieval, cumulus cells were immediately removed and then ICSI was performed; in the second group, the oocyte-cumulus complexes were incubated for 4 hours. Afterwards, the granulosa cells were removedand ICSI was immediately performed. |
| Measure | Description | Time Frame |
|---|---|---|
| Number of M2 oocytes at injection | Asessment of the number of M2 oocytes was made after the oocyte denudation and ICSI | four hours after oocyte retrieval |
| Number of normal fertilized oocytes after ICSI | Asessment of the normal fertilization rate was made 24 hours after the intervention. | 24 hours |
| Number of cleaving embryos | The embryo cleavage was evaluated after 48 hours and 72 hours after the ICSI was performed. | 72 hours |
| Number of TOP embryos | The embryos were morfologically classified into 4 categories . It considered TOP embryos those correctly fertilized at day 1, and with regular 6 to 8 cells at day 2 with less than 20% fragmentation and without multinuclear blastomeres. | 72 hours |
| Measure | Description | Time Frame |
|---|---|---|
| Implantation rate | The implantation rate was the ratio between the number of gestacional sacs and the number of transferred embryos. | 14 days after embryo trasfer |
| Clinical pregnancy rate | Clinical pregnancy is confirmed by ultrasonography ,when one or more gestational sacs with heart activity are seen. |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Romina I Pesce, MD | Hospital Italiano de Buenos Aires | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Hospital Italiano de Buenos Aires | Ciudad de Buenos Aires | Buenos Aires | C1199ABB | Argentina |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 3294045 | Background | Harrison KL, Wilson LM, Breen TM, Pope AK, Cummins JM, Hennessey JF. Fertilization of human oocytes in relation to varying delay before insemination. Fertil Steril. 1988 Aug;50(2):294-7. doi: 10.1016/s0015-0282(16)60076-6. | |
| 11473969 | Background | Jacobs M, Stolwijk AM, Wetzels AM. The effect of insemination/injection time on the results of IVF and ICSI. Hum Reprod. 2001 Aug;16(8):1708-13. doi: 10.1093/humrep/16.8.1708. |
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| 6 to 7 week |
| 9619563 | Result | Rienzi L, Ubaldi F, Anniballo R, Cerulo G, Greco E. Preincubation of human oocytes may improve fertilization and embryo quality after intracytoplasmic sperm injection. Hum Reprod. 1998 Apr;13(4):1014-9. doi: 10.1093/humrep/13.4.1014. |
| 9853875 | Result | Van de Velde H, De Vos A, Joris H, Nagy ZP, Van Steirteghem AC. Effect of timing of oocyte denudation and micro-injection on survival, fertilization and embryo quality after intracytoplasmic sperm injection. Hum Reprod. 1998 Nov;13(11):3160-4. doi: 10.1093/humrep/13.11.3160. |
| 9756300 | Result | Yanagida K, Yazawa H, Katayose H, Suzuki K, Hoshi K, Sato A. Influence of oocyte preincubation time on fertilization after intracytoplasmic sperm injection. Hum Reprod. 1998 Aug;13(8):2223-6. doi: 10.1093/humrep/13.8.2223. |
| 14531646 | Result | Ho JY, Chen MJ, Yi YC, Guu HF, Ho ES. The effect of preincubation period of oocytes on nuclear maturity, fertilization rate, embryo quality, and pregnancy outcome in IVF and ICSI. J Assist Reprod Genet. 2003 Sep;20(9):358-64. doi: 10.1023/a:1025476910771. |
| 11699125 | Result | Hassan HA. Cumulus cell contribution to cytoplasmic maturation and oocyte developmental competence in vitro. J Assist Reprod Genet. 2001 Oct;18(10):539-43. doi: 10.1023/a:1011901923261. |