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To assess the impact of the female genital microbiome on ART outcome.
The main objective of this study is to learn more about which microbial spectra are negatively or positively impacting IVF pregnancy outcome. This information is needed before proceeding to the next step in which an adaptation of the microbiome can be attempted to improve IVF outcome. The study is expected to shed light over the possible influence of the microbiome on causes for subfertility, endometrial receptivity and obstetrical complications. A diagnostic link may be found with entities that are often unexplained today, e.g. (recurrent) miscarriage and repeated implantation failure.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Study arm | Women in the study arm will undergo microbiome samplings described in the interventions section (3 sampling points) |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| 3 Sampling points | Other | When entering the in-vitro fertilization/intracytoplasmatic sperm injection 4 samples will be harvested: a rectal swab, a cervical swab, a vaginal swab and an intra-uterine sample using an empty embryo catheter. After the ovarian stimulation treatment, the same patients will be sampled two more times: once at the moment of oocyte retrieval (rectal, cervical, vaginal and intra-uterine samples) and again just before the embryo transfer (at this stage, the embryo culture medium will be harvested, as well as an intra-uterine sample using the tip of an empty embryo inner catheter before the embryo transfer and a rectal sample) |
| Measure | Description | Time Frame |
|---|---|---|
| Clinical pregnancy | To determine if there Is there a association between the microbiome profile and pregnancy outcome after ART | 7 weeks |
| Measure | Description | Time Frame |
|---|---|---|
| Baseline microbiome profile | Descriptive analysis of the microbiome present in women suffering of infertility | 1 week |
| Variation of microbiome profile during assisted reproductive technologies |
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Inclusion Criteria:
Exclusion Criteria:
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Women undergoing in-vitro fertilization
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| Name | Affiliation | Role |
|---|---|---|
| Christophe Blockeel, PhD | Clinical head | Study Director |
| Julie Bussche, Msc | Research collaborator | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Universitair Ziekenhuis Brussel | Brussels | 1090 | Belgium |
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| Label | URL |
|---|---|
| Principal research site | View source |
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| ID | Term |
|---|---|
| D007246 | Infertility |
| ID | Term |
|---|---|
| D000091662 | Genital Diseases |
| D000091642 | Urogenital Diseases |
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Microbiome analysis using 16S ribosomal RNA sequencing
|
Descriptive analysis of the microbiome present in women suffering of infertility according to the assisted reproductive technologies cycle stage
| 5 weeks |
| Microbiome profile of the embryo culture | Descriptive analysis of the microbiome present in the embryo culture on the day of embryo transfer | 1 week |
| Live birth | To determine if there Is there a association between the microbiome profile and pregnancy outcome after assisted reproductive technologies | 43 weeks |