The primary goal of this study is to test the effects of a high-fat, high-carbohydrate mixed meal on candidate modulators of cardiovascular disease risk (inflammation, vascular reactivity, sympathetic nervous system tone, and response to pain) in young adult women with migraine compared with healthy young women. This is study enrolling both cases (women with migraine) and controls (women without migraine), with equal numbers of normal weight and obese women in each group. Participants will undergo a telephone screening and a single day in-person study visit.

Inclusion Criteria (all):

• Female 18-35 years of age, inclusive
• BMI 18.5-24.9 kg/m2, inclusive
• BMI ≥30 kg/m2

Inclusion Criteria (cases only):

• Diagnosis of migraine per ICHD-3-beta (1-10 migraine days per month)

Exclusion Criteria (all):

• Smoking (current or former)
• Chronic use of medications, including oral contraceptives
• Major medical or psychiatry illness (e.g. diabetes, fibromyalgia, major depression/anxiety)
• Pregnant or nursing
• Allergies or sensitivity to any of the ingredients of the meal
• Inability to fast for 10 hours and/or abstain from caffeine intake for 24 hours
• Treatment with antibiotics or steroids within the previous 3 months
• Treatment with NSAIDs within the previous 1 week

Exclusion Criteria (controls only):

• Migraine headaches, or headaches other than tension-type headaches with a frequency of 1 or fewer days per month

Self-reported female.

After the primary publication is complete, interested investigators can request these data without identifiers. The study team will evaluate the request for scientific rigor and ensure consistency with our protocol, and then provide data without identifiers via secure file-share. This will include the subset of participants who indicated their data could be used for ancillary and/or future studies.