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| ID | Type | Description | Link |
|---|---|---|---|
| 2016-A01025-46 | Other Identifier | ID-RCB |
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| Name | Class |
|---|---|
| ANRS, Emerging Infectious Diseases | OTHER_GOV |
| Institut National de la Santé Et de la Recherche Médicale, France | OTHER_GOV |
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The objective of our project is to determine the infected seminal cell types and the molecular mechanisms involved in HIV cell-associated transmission in the colo-rectal mucosa, using conditions as close as possible to real life, i.e. seminal leukocytes and seminal plasma from HIV-infected donors and tissue explants
This project relies on:
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| HIV-1 infected patients | Other | Blood sample and sperm sample are collected |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| blood sample | Other | A sample of HIV-1 infected patients's blood is collected |
| |
| Measure | Description | Time Frame |
|---|---|---|
| Characterize seminal HIV infected cells. | Macrophages and CD4+ T lymphocytes characterization. | 1 years |
| Evaluate the potential of seminal HIV infected cells migration and adhesion. | Assess the potential of seminal macrophages and CD4 T cells to adhere and transmigrate. | 1 years |
| Determine the efficiency of seminal cell associated infection. | The efficiency of seminal cell associated infection will be determinated in the colo-rectal explants. | 1 years |
| Measure | Description | Time Frame |
|---|---|---|
| Identify molecules expressed and involved in seminal cell adhesion and transmigration. | Identify key adhesion molecules expressed by seminal leucocytes and epithelial cells involved in seminal cell adhesion/transmigration. | 1 years |
| Identify seminal and mucosal molecules mediating seminal cell translocation. |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Nathalie Dejucq-Rainsford, PhD | IRSET | Study Director |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| CECOS Bretagne Rennes | Rennes | Brittany Region | 35200 | France | ||
| CECOS Hôpital Paule de Viguier |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 17148672 | Background | Roulet V, Satie AP, Ruffault A, Le Tortorec A, Denis H, Guist'hau O, Patard JJ, Rioux-Leclerq N, Gicquel J, Jegou B, Dejucq-Rainsford N. Susceptibility of human testis to human immunodeficiency virus-1 infection in situ and in vitro. Am J Pathol. 2006 Dec;169(6):2094-103. doi: 10.2353/ajpath.2006.060191. | |
| 18347738 | Background |
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The duplicated part of the CRFs will then be sent to Mrs DEJUC at the IRST in Rennes, the remaining part will be kept in the investigating center.
All the data of the study transcribed by the investigator in the CRFs will be transmitted electronically to the person in charge of the data of the INSERM / IRSET 1085 unit. Upon receipt, the data will be entered simply by typing and re-reading on the Excel spreadsheet. The files will be locked by password and backed up daily with storage for 3 months on the server of the INSERM 1085 unit managed by the IT department of the University of Rennes 1. The set Data is saved every evening, with storage for 4 weeks, then archived monthly on tape.
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| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
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| sperm sample |
| Other |
A sample of HIV-1 infected patients's sperm is collected |
|
Seminal and mucosal soluble molecules mediating seminal cell translocation will be characterized (passive diffusion or active transmigration). |
| 1 years |
| Determine the impact of seminal plasma/cells exposure on mucosal immune cell. | Mucosal immune cell activation, trafficking and the factors involved, will be evaluated. | 1 years |
| Toulouse |
| Midi-Pyrénées |
| 31059 |
| France |
| CECOS Paris Bichat | Paris | 75018 | France |
| Le Tortorec A, Le Grand R, Denis H, Satie AP, Mannioui K, Roques P, Maillard A, Daniels S, Jegou B, Dejucq-Rainsford N. Infection of semen-producing organs by SIV during the acute and chronic stages of the disease. PLoS One. 2008 Mar 12;3(3):e1792. doi: 10.1371/journal.pone.0001792. |
| 19117522 | Background | Le Tortorec A, Satie AP, Denis H, Rioux-Leclercq N, Havard L, Ruffault A, Jegou B, Dejucq-Rainsford N. Human prostate supports more efficient replication of HIV-1 R5 than X4 strains ex vivo. Retrovirology. 2008 Dec 31;5:119. doi: 10.1186/1742-4690-5-119. |
| 25414416 | Background | Houzet L, Matusali G, Dejucq-Rainsford N. Origins of HIV-infected leukocytes and virions in semen. J Infect Dis. 2014 Dec 15;210 Suppl 3:S622-30. doi: 10.1093/infdis/jiu328. |
| 26854806 | Background | Camus C, Matusali G, Bourry O, Mahe D, Aubry F, Bujan L, Pasquier C, Massip P, Ravel C, Zirafi O, Munch J, Roan NR, Pineau C, Dejucq-Rainsford N. Comparison of the effect of semen from HIV-infected and uninfected men on CD4+ T-cell infection. AIDS. 2016 May 15;30(8):1197-208. doi: 10.1097/QAD.0000000000001048. |
| 20010071 | Background | Anderson DJ, Politch JA, Nadolski AM, Blaskewicz CD, Pudney J, Mayer KH. Targeting Trojan Horse leukocytes for HIV prevention. AIDS. 2010 Jan 16;24(2):163-87. doi: 10.1097/QAD.0b013e32833424c8. No abstract available. |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |