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This study aims to assess the phenotype of the enteric nervous system (enteric neurons) in healthy subjects.
The enteric nervous system (ENS) is composed by enteric neurons and enteric glial cells. There is a cross-talk between ENS and the intestinal epithelial barrier (IEB). ENS and IEB together constitute the neuronal-glial-epithelial unit. This unit has a key role in gut functions. The characteristics and the phenotype of the ENS also change according to age and environmental factors. Similar study is ongoing for patients with Spina Bifida. Anorectal data and phenotype of the neuronal-glial-epithelial unit in healthy subjects is required to assess abnormality of these items in patients with neurological disease including Spina Bifida.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Healthy Subjects | Other |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| endoscopy | Procedure | colonic biopsies during endoscopy and anal manometry with barostat and anal endoflip |
|
| Measure | Description | Time Frame |
|---|---|---|
| Number of enteric neurons per ganglion | colonic biopsies will be used to study intestinal permeability, colonic inflammation and enteric nervous system | data collected at the day of endoscopy |
| Measure | Description | Time Frame |
|---|---|---|
| density and activity of the enteric glial cells | colonic biopsies will be used to study intestinal permeability, colonic inflammation and enteric nervous system | data collected at the day of endoscopy |
| rectal adaptation |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Charlène BROCHARD, Md | Rennes University Hospital | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Rennes University Hospital | Rennes | 35033 | France |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 37803037 | Derived | Lefevre C, Le Roy C, Bessard A, Le Berre-Scoul C, Marchix J, Coron E, Le Rhun M, Brochard C, Perrouin-Verbe B, Neunlist M. Region-specific remodeling of the enteric nervous system and enteroendocrine cells in the colon of spinal cord injury patients. Sci Rep. 2023 Oct 6;13(1):16902. doi: 10.1038/s41598-023-44057-y. | |
| 35505001 | Derived |
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| ID | Term |
|---|---|
| D004724 | Endoscopy |
| ID | Term |
|---|---|
| D003949 | Diagnostic Techniques, Surgical |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
| D019060 | Minimally Invasive Surgical Procedures |
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rectal adaptation is defined by a volume variation during ascending pressure (phasic distension). It will be performed with a bag connected to electronic barostat
| data collected at the day of endoscopy |
| rectal perception | It will performed by using 6-point Likert scale ranging from 0 (no sensation) to 6 (intolerable pain) | data collected at the day of endoscopy |
| anal distensiblity | It will be performed by using ENDOFLIP | data collected at the day of endoscopy |
| intestinal permeability | Biopsy samples collected at the time of colonoscopy will be use within a Ussing Chamber able to assess intestinal permeability. The Ussing chamber is used to measure the short-circuit current as an indicator of net ion transport taking place across an epithelium, such as gut mucosa. The measure will be performed on 2 biopsies from each patient. | data collected at the day of endoscopy |
| macroscopic and microscopic colonic inflammation | Colonic inflammation will be assessed during the scope procedure. The physician will assess the normality of the mucosa or not in case of mucosal erythema, ulceration or a decrease of the vascular pattern. Microscopic inflammation will be assessed by the pathologist according to neutrophils infiltration in the gut, crypt abscess, crypt distortion. | data collected at the day of endoscopy |
| Brochard C, Bouguen G, Olivier R, Durand T, Henno S, Peyronnet B, Pagenault M, Lefevre C, Boudry G, Croyal M, Fautrel A, Esvan M, Ropert A, Dariel A, Siproudhis L, Neunlist M. Altered epithelial barrier functions in the colon of patients with spina bifida. Sci Rep. 2022 May 3;12(1):7196. doi: 10.1038/s41598-022-11289-3. |
| D013514 | Surgical Procedures, Operative |