The purpose of this study is to evaluate the efficacy and safety of human amniotic epithelial cells transplant in nonunion of limb fracture patients.

In this study, patients of nonunion after limb fracture will be treated with human amniotic epithelial cells. Clinical and radiological assessment is performed.

hAECs developing from the epiblast as early as 8 days after fertilization, recent data reported indicate that hAECs possess proper osteogenic differentiation potential and a localized modulatory influence on the early tissue remodeling process, making these cells a promising stem cell source for bone regeneration after fractures.

Inclusion Criteria:

• diagnosed with nonunion of limbs by x-ray and bone scan;
• compliance to treatment and long term follow-up;
• be able and willing to participate in the study;
• written informed consent before study.

Exclusion Criteria:

• pregnant or breastfeeding;
• patients with malignancy, immunocompromised ( HIV AIDS, Diabetes mellitus, active Hepatitis), in a immunosuppressant therapy ( chemotherapy or steroids), OR health status, mental diseases, which will influence the study;
• patients undergoing any other treatment that are not related to this study;
• others considered not appropriate selected patients.