AvenovaTM as a Sterile Skin Preparation Agent

Comparison of Antimicrobial Activity of AvenovaTM as a Sterile Skin Preparation in Humans

Aim 1: To determine in vivo capability of AvenovaTM against common cutaneous microbial biome on human skin

Aim 2: To compare to povidone-iodine 5% solution, 4% chlorhexidine and isopropyl alcohol against AvenovaTM as a sterile skin agent

A total of approximately 30 eligible participants will be identified with clinically-acquired information by Dr. Wendy Lee, an oculoplastic surgeon and aesthetic treatment specialist at Bascom Palmer Eye Institute, during regular clinic visits.

Part One: Patients will have their skin swabbed and then cultured for various skin flora agents. The petri dish will then be swabbed with Avenova to see what percent of skin flora microbe is killed.

Part Two: Patients will have their skin divided into 4 quadrants. The area will be pre-swabbed to monitor microbe growth. Each of 4 quadrants will be cleansed for one minute with AvenovaTM , povidone-iodine 5% solution, 4% chlorhexidine and isopropyl alcohol, then swabbed to monitor for microbe growth.

The swabs will be placed in thioglycollate broth to monitor for bacterial growth. If growth if observed in the thioglycollate, the samples will be cultured on blood agar plates and the bacterial specie(s) identified.

Inclusion Criteria:

• Adults age 18 and above able to provide informed consent to participate Adults attending Dr. Wendy Lee Oculoplastics Clinic

Exclusion Criteria:

• Adults unable to consent
• Individuals who less than 18 years of age
• Prisoners
• Pregnant women - while these cleansing agents are used on pregnant women as standard cleansing agents, effects to fetus unknown
• Patients currently using oral or topical antimicrobial agents
• History of skin infection to facial injectable of surgery
• Inability to tolerate cleansing procedure
• Inability to sit comfortably for 15 - 30 minutes