We are looking to see how the addition of nitrosomonas alters the presence of nitrolipids and inflammatory cytokines on the skin.

We are looking to see how the nitrosomonas alters nitrolipids in the skin and the inflammatory cytokines of the skin.

We will be using healthy subjects over the course of 2 weeks.

Subjects will be using a neutral cleanser for one week prior to the study, skin microbiome,facial photography, lipid and cytokine measurements will be taken at baseline. Subjects will then start using nitrosomonas spray twice daily for one week.

There is a portion of the study where the subjects will have skin microbiome, facial photography, lipid measurements, blood pressure measurements and a bloow draw before and after using the nitrosomonas spray for one week.

Inclusion Criteria:

• Those aged 18 and over
• Healthy Subjects with clear skin

Exclusion Criteria:

• Adults unable to consent
• Individuals who are not yet adults (infants, children, teenagers)
• Pregnant women
• Prisoners
• Those with acne, eczema, seborrheic dermatitis, rosacea or polycystic ovarian syndrome
• Those who have started new oral medication in the last four weeks
• Those who have used systemic antibiotics in the last month
• Those who have used isotretinoin in the last 6 weeks
• Those who have used topical antibiotics or retinoid in the last two weeks.
• Those who have autoimmune or metabolic diseases
• Those who have changed brands of oral contraceptive within the last four weeks
• Those who have chronic medical disorders
• Those whose with active skin infections
• Concomitant use of nitrates
• Concomitant use of anti-hypertensive agents
• Those with syncopal episodes