| Primary | Volar Forearm Sweat Lipid Mediator Concentrations Following Pilocarpine Stimulation | Approximately 150 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids and endocannabinoid-like molecules, and sphingolipids. | Of the 14 subjects recruited for this study, only 7 provided usable data for this primary outcome. | Posted | | Mean | Standard Deviation | picomoles per milliliter | | measured at study visit 1, 2 and 3; detected lipid mediator concentrations for study visit 1 reported | | | | ID | Title | Description |
|---|
| OG000 | Single Group | All subjects will participate in 4 study day visits in the same order.
- compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).
- compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).
- collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).
- collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.
Ibuprofen: 400 mg ibuprofen given to inhibit COX metabolism Physiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating Pilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine |
| | | Title | Denominators | Categories |
|---|
| 9-HODE | | | | 13-HODE | | | | 9-HOTrE | | |
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| Primary | Volar Forearm Sweat Lipid Mediator Concentrations Following Exercise | Approximately 150 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids and endocannabinoid-like molecules, and sphingolipids. | Of the 14 subjects recruited for this study, only 7 provided usable data for this primary outcome. | Posted | | Mean | Standard Deviation | picomoles per milliliter | | measured at study visit 1, detected lipid mediator concentrations reported | | | | ID | Title | Description |
|---|
| OG000 | Single Group | All subjects will participate in 4 study day visits in the same order.
- compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).
- compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).
- collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).
- collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.
Ibuprofen: 400 mg ibuprofen given to inhibit COX metabolism Physiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating Pilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine |
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| Primary | Lower Back Sweat Lipid Mediator Concentrations Following Pilocarpine Stimulation | Approximately 150 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids and endocannabinoid-like molecules, and sphingolipids. | Of the 14 subjects recruited for this study, only 4 provided usable data for this primary outcome. | Posted | | Mean | Standard Deviation | picomoles per milliliter | | measured at study visit 1, detected lipid mediator concentrations reported | | | | ID | Title | Description |
|---|
| OG000 | Single Group | All subjects will participate in 4 study day visits in the same order.
- compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).
- compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).
- collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).
- collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.
Ibuprofen: 400 mg ibuprofen given to inhibit COX metabolism Physiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating Pilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine |
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| Primary | Anterior Distal Thigh Sweat Lipid Mediator Concentrations Following Pilocarpine Stimulation | Approximately 150 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids and endocannabinoid-like molecules, and sphingolipids. | Of the 14 subjects recruited for this study, only 4 provided usable data for this primary outcome. | Posted | | Mean | Standard Deviation | picomoles per milliliter | | measured at study visit 2, detected lipid mediator concentrations reported | | | | ID | Title | Description |
|---|
| OG000 | Single Group | All subjects will participate in 4 study day visits in the same order.
- compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).
- compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).
- collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).
- collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.
Ibuprofen: 400 mg ibuprofen given to inhibit COX metabolism Physiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating Pilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine |
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| Primary | Change in Plasma Lipid Mediator Concentrations Before and After Oral Ibuprofen Administration | Approximately 100 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids, and endocannabinoid-like molecules. | Of the 14 subjects recruited for this study, only 9 provided usable data for this primary outcome. | Posted | | Geometric Mean | Full Range | picomoles per milliliter | | measured at four timepoints at study visit 4, detected lipid mediator concentrations for first timepoint reported | | | | ID | Title | Description |
|---|
| OG000 | Single Group | All subjects will participate in 4 study day visits in the same order.
- compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).
- compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).
- collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).
- collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.
Ibuprofen: 400 mg ibuprofen given to inhibit COX metabolism Physiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating Pilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine |
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| Secondary | Change in Pilocarpine-stimulated Sweat Lipid Mediator Concentrations Before and After Oral Ibuprofen Administration | Approximately 100 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids, and endocannabinoid-like molecules. | Of the 14 subjects recruited for this study, only 9 provided usable data for this primary outcome. | Posted | | Geometric Mean | Full Range | picomoles per milliliter | | measured at four timepoints at study visit 4, detected lipid mediator concentrations for first timepoint reported | | | | ID | Title | Description |
|---|
| OG000 | Single Group | All subjects will participate in 4 study day visits in the same order.
- compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).
- compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).
- collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).
- collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.
Ibuprofen: 400 mg ibuprofen given to inhibit COX metabolism Physiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating Pilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine |
| |
| Secondary | Change in Plasma Ibuprofen Concentrations | Plasma concentration of ibuprofen prior to and 30 min, 2 hr and 4 hr after oral administration | Of the 14 subjects recruited for this study, only 9 provided usable data for this primary outcome. | Posted | | Geometric Mean | Full Range | micrograms per milliliter | | measured at four timepoints at study visit 4 | | | | ID | Title | Description |
|---|
| OG000 | Single Group | All subjects will participate in 4 study day visits in the same order.
- compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).
- compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).
- collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).
- collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.
Ibuprofen: 400 mg ibuprofen given to inhibit COX metabolism Physiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating Pilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine |
| |
| Secondary | Change in Sweat Ibuprofen Concentrations | Sweat concentration of ibuprofen prior to and 30 min, 2 hr and 4 hr after oral administration | Of the 14 subjects recruited for this study, only 9 provided usable data for this primary outcome. | Posted | | Geometric Mean | Full Range | micrograms per milliliter | | measured at four timepoints at study visit 4 | | | | ID | Title | Description |
|---|
| OG000 | Single Group | All subjects will participate in 4 study day visits in the same order.
- compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).
- compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).
- collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).
- collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.
Ibuprofen: 400 mg ibuprofen given to inhibit COX metabolism Physiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating Pilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine |
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