Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Class |
|---|---|
| Anti-Diabetic Food Centre | OTHER |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
This study aims to investigate the effect of mixed of herbs and spices on gene expression of pathways associated with glucose homeostasis, oxidative stress, inflammation and its interrelation with circulating miRNA, in the postprandial phase in healthy human subjects.
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| High fat challenge breakfast | Placebo Comparator | High fat breakfast without mixed-spices (% Energy Carbohydrate:Fat:Prot / 20:60:20), acute study / one time administration |
|
| High fat challenge breakfast with mixed-spices | Experimental | High fat breakfast with mixed-spices (% Energy Carbohydrate:Fat:Prot / 20:60:20), acute study / one time administration |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| High fat challenge breakfast | Other | In this intervention, subjects are asked to consume high fat breakfast without mixed-spices. Standardized high fat breakfast corresponding to % Energy of Carbohydrate:Fat:Prot / 20:60:20 |
| Measure | Description | Time Frame |
|---|---|---|
| Change from Baseline (0 hr) in untargeted PBMC gene expression profile at 4 hr | The venous blood samples will be taken for isolation of peripheral blood mononuclear cells. Extracted total RNA will then be used for running RNA-microarray (Illumina, San Diego, CA, USA) | Prior to the initial intervention at 0 hr and after intervention at 4 hr |
| Measure | Description | Time Frame |
|---|---|---|
| Postprandial appetite ratings after acute intervention | Appetite will be assessed using standard subjective 100 mm VAS (visual analogue scale) at respective time frame throughout each visit. Appetite questionnaire includes following questions: How hungry do you feel?, How strong is your desire to eat?, How satiated (i.e. pleasantly satisfied) are you?, How full do you feel?, How much food do you think you can (or would want to) eat?, How thirsty are you? |
Not provided
Inclusion Criteria:
Exclusion Criteria:
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Affiliation | Role |
|---|---|---|
| Yoghatama Cindya Zanzer, MSc PhDcand | Lund University | Principal Investigator |
| Elin Östman, PhD A/Prof | Lund University | Principal Investigator |
| Congyu Xue, BSc | Lund University | Study Chair |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Food for Health Science Centre, Lund University | Lund | Skåne County | 22381 | Sweden |
Not provided
| Label | URL |
|---|---|
| Antidiabetic Food Centre - VINNOVA Excellence Centre in Research and Innovation at Lund University, SWEDEN | View source |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| High fat challenge breakfast with mixed-spices | Other | In this intervention, subjects are asked to consume high fat breakfast with mixed-spices. Standardized high fat breakfast corresponding to % Energy of Carbohydrate:Fat:Prot / 20:60:20 |
|
| Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 and 240 min. |
| Postprandial gastrointestinal tolerance ratings after acute intervention | Gastrointestinal tolerance will be assessed using standard subjective 100 mm VAS (visual analogue scale) at respective time frame throughout each visit. Gastrointestinal tolerance questionnaire includes following questions: Do you experience any abdominal pain?, Do you experience any rumbling noises in your stomach?, Do you experience any flatulence (generation of excessive gas)?, Do you experience any bloated (swollen) feeling in the abdomen?, Do you experience any acid reflux (heartburn)?, Do you experience nausea?, Do you experience an urge to vomit? | Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 and 240 min. |
| Postprandial blood glucose after acute intervention | The capillary blood samples will be taken for blood glucose analysis. | Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 and 240 min. |
| Postprandial insulin after acute intervention | The venous blood samples will be taken for insulin analysis. Insulin analysis will be conducted with routine hospital analysis at Labmedicine Skåne. | Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 and 240 min. |
| Postprandial triacylglycerol after acute intervention | The venous blood samples will be taken for plasma triacylglycerol analysis. Plasma triacylglycerol analysis will be conducted with routine hospital analysis at Labmedicine Skåne. | Prior to the initial intervention at 0 min and after intervention at 30, 60, 90, 120, 180 and 240 min. |
| Postprandial non-esterified fatty acids (NEFA) after acute intervention | The venous blood samples will be taken for serum NEFA analysis. Serum NEFA analysis will be conducted with routine hospital analysis at Labmedicine Skåne. | Prior to the initial intervention at 0 min and after intervention at 30, 60, 90, 120, 180 and 240 min. |
| Postprandial total cholesterol after acute intervention | The venous blood samples will be taken for plasma total cholesterol analysis. Plasma total cholesterol analysis will be conducted with routine hospital analysis at Labmedicine Skåne. | Prior to the initial intervention at 0 min and after intervention at 30, 60, 90, 120, 180 and 240 min. |
| Postprandial high density lipoprotein (HDL)-cholesterol after acute intervention | The venous blood samples will be taken for plasma HDL-cholesterol analysis. Plasma HDL-cholesterol analysis will be conducted with routine hospital analysis at Labmedicine Skåne. | Prior to the initial intervention at 0 min and after intervention at 30, 60, 90, 120, 180 and 240 min. |
| Postprandial low density lipoprotein (LDL)-cholesterol after acute intervention | The venous blood samples will be taken for plasma LDL-cholesterol analysis. Plasma LDL-cholesterol analysis will be conducted with routine hospital analysis at Labmedicine Skåne. | Prior to the initial intervention at 0 min and after intervention at 30, 60, 90, 120, 180 and 240 min. |
| Postprandial interleukin (IL)-6 after acute intervention | The venous blood samples will be taken for serum interleukin IL-6 analysis. Serum IL-6 analysis will be conducted with routine hospital analysis at Labmedicine Skåne. | Prior to the initial intervention at 0 min and after intervention at 120 and 240 min. |
| Postprandial interleukin (IL)-10 after acute intervention | The venous blood samples will be taken for serum interleukin IL-10 analysis. Serum IL-10 analysis will be conducted with routine hospital analysis at Labmedicine Skåne. | Prior to the initial intervention at 0 min and after intervention at 120 and 240 min. |
| Postprandial tumor necrosis factor (TNF)-alpha after acute intervention | The venous blood samples will be taken for serum TNF-alpha analysis. Serum TNF-alpha analysis will be conducted with routine hospital analysis at Labmedicine Skåne. | Prior to the initial intervention at 0 min and after intervention at 120 and 240 min. |
| Postprandial lipid peroxidation (MDA equivalent) after acute intervention | The venous blood samples will be taken for lipid peroxidation (MDA) assay. | Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 and 240 min. |
| Postprandial total antioxidant capacity after acute intervention | The venous blood samples will be taken for total antioxidant capacity (FRAP) assay. | Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 and 240 min. |
| Change from Baseline (0 hr) in untargeted circulating miRNA profile at 4 hr | The venous blood samples will be taken for isolation of peripheral blood mononuclear cells. Extracted total RNA will then be used for running miRNA (Affymetrix, Santa Clara, CA, USA) | Prior to the initial intervention at 0 hr and after intervention at 4 hr |
| ID | Term |
|---|---|
| D024821 | Metabolic Syndrome |
| D007249 | Inflammation |
| ID | Term |
|---|---|
| D007333 | Insulin Resistance |
| D006946 | Hyperinsulinism |
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
Not provided
Not provided