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The GASTROVIM research explores the links between individual genetic susceptibility, genetic variability of rotavirus strains and effectiveness of immunization with the rotavirus vaccination: a clinical investigation to assess glycan attachment specificity, toward rotavirus vaccine improvement.
The work will be carried out in France and in a tropical area of population with diverse geographical origins: the Guyana populated with Native Americans, people of European descent, people of Asian (Hmong) and a large source population African.
The first aim of the project will be to characterize the specificity of the VP8 * HBGA of RotaTeq and Rotarix vaccines in order to ensure their binding characteristics glycans are similar to those of recent P8 clinical strains circulating in France and were maintained in the culture passages.
The second objective will be to validate the impact of recently described polymorphisms HBGAs on susceptibility to infection by RVA through GASTROVIMc prospective clinical research.
The third objective will be to determine the relative role of recognition and HBGAs ganglioside in the initial attachment and viral entry.
The expression of HBGAs and ganglioside by permissive cell lines in culture human stem RVA be manipulated to define the role of glycans in attachment and infection.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Case group | 250 children from Nantes University Hospital and 150 children from Guyana Hospital admitted in paediatric emergency unit for diagnosed rotavirus acute gastroenteritis: rotavirus rapid screen test and oral swab for polymorphism exploration |
| |
| Control group | 250 children from Nantes University Hospital and 150 children from Guyana Hospital admitted in paediatric emergency unit without signs of infection and / or digestive clinical picture evocative of gastroenteritis. Paired with a case upon ethnicity or origin if impossible to match the ethnicity of the case. oral swab for polymorphism exploration |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| rotavirus rapid screen test | Biological | According to routine care |
| |
| Measure | Description | Time Frame |
|---|---|---|
| Absence / Presence of non-secretory polymorphisms FUT2- and Lewis Negative FUT3- | 6 months after inclusion |
| Measure | Description | Time Frame |
|---|---|---|
| Population frequency of new protective genotypes to rotavirus infection | Six months after inclusion | |
| Absence / presence of genetic polymorphisms --other (Including FUT2- and FUT3-) | Six months after inclusion |
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Inclusion Criteria:
Exclusion Criteria:
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400 children admitted to the pediatric emergency for acute gastroenteritis (cases) and 400 children admitted to the pediatric emergency without evocative picture of infection or gastroentieritis
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| Name | Affiliation | Role |
|---|---|---|
| Christèle GRAS-LEGUEN, MD PhD | Nantes University Hospital | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Nantes University Hospital | Nantes | Loire-Atlantique | 44093 | France |
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| ID | Term |
|---|---|
| D005759 | Gastroenteritis |
| ID | Term |
|---|---|
| D005767 | Gastrointestinal Diseases |
| D004066 | Digestive System Diseases |
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Oral swab
| polymorphism exploration |
| Genetic |
from an oral swab collected after parents consent |
|
| Absence / presence of different viral strains Rotavirus (RVA) involved in the acute gastroenteritis episodes in French Guiana | Six months after inclusion |
| Absence / presence of various HBGA polymorphisms involved in acute gastroenteritis episodes in French Guiana | Six months after inclusion |
| population frequency of different etiologies of acute gastroenteritis viral, bacterial and / or parasitic (Cayenne) | Six months after inclusion |