Cohort Biomonitoring Study to Evaluate the Biocompatibilty of Resin-based Dental Materials

Cohort Biomonitoring Study to Evaluate the Biocompatibilty of Resin-based Dental Materials

Adult patients who require multiple dental restorations will be included in this study. Urine and saliva samples will be taken for determination of bisphenol A levels at several time points, and for analysis of the estrogenic activity using ERE-CALUX.

Also buccal cells will be taken. Next, DNA extraction will be done with a commercial kit. We will determine global alterations in DNA methylation and DNA hydroxymethylation levels using UPLC-MS/MS.

Inclusion Criteria:

• Caucasian, adults,

Exclusion Criteria:

• acute or chronic medical condition, presence of oral inflammatory conditions during the previous 2 weeks, chronic disease requiring intake of drugs, exposure to diagnostic X-rays in the previous 2 months, smoking and consumption of alcohol