Background:

Functional hypothalamic amenorrhea (functional HA) is a condition where a woman s period stops for a temporary time. This is due to improper function of the hypothalamus. This is the part of the brain that directs the whole reproductive system. Researchers want to learn more about functional HA. They also want to learn how diet, exercise, and other factors may change women s menstrual cycles.

Objective:

To better understand functional HA.

Eligibility:

Healthy women ages 18-28 years old who:

• Have regular periods
• Exercise no more than 4 hours a week
• Had their first period at age 11-14

Design:

Participants will be prescreened over the phone.

Participants will be screened with:

• Blood and urine tests
• Medical history
• Physical exam.

Participants will have 9 or 10 visits over about 3 menstrual cycles. These include:

• Repeat of screening tests
• Questionnaires
• Exercise test
• Resting energy expenditure test: Participants fast overnight before the test. They lie on their back under a canopy for a half hour.
• Body composition test: This is done with a dual energy x-ray absorptiometry (DXA) scan.
• Pelvic ultrasound
• For two full-day visits, an IV is inserted into an arm vein. The IV takes a blood sample every 10 minutes for 8 hours.

Participants will keep logs:

• Menstrual cycle log
• Diet log for three 4-day cycles

Participants will receive test kits to complete at home:

• Daily blood and urine sample
• Ovulation

Participants will take a daily iron supplement. They will wear a wristband that monitors activity 24 hours a day.

Participants will stick to a special diet for two 5-day periods of time. They will complete two 4-day exercise programs....

Functional hypothalamic amenorrhea (HA) is a reversible form of hypogonadotropic hypogonadism (HH) that can be triggered by stressors such as exercise, nutritional deficits, and psychological stress. Dysfunction of the hypothalamic component of the reproductive axis plays a key role in functional HA and is manifest by an altered pattern of luteinizing hormone (LH) pulses detectable in peripheral blood. There is ample evidence supporting the use of LH as a surrogate marker of hypothalamic gonadotropin-releasing hormone (GnRH) secretion from the hypothalamus. There is also significant evidence that women vary in their susceptibility to such stress-induced amenorrhea, pointing to a role for both environmental and genetic factors in the etiology of functional HA. However, the variation in changes in GnRH pulse frequency in response to stressors in healthy women has not been defined. Data from previous work in our lab has suggested that rare variants in genes associated with other forms of HH may also contribute to the variability seen in susceptibility to functional HA. The long-term goal of our research is to examine the interaction of environment and genes in HA. In this pilot study we propose to examine the inter-individual variability in pulsatile LH secretion in response to standardized neutral and deficient energy availability (NEA and DEA, respectively) in normal women. We will then relate this primary end-point to proposed predictive factors including past reproductive and family history and markers of current metabolic status and their response to energy availability. Our initial analyses will help to determine simplified biomarkers that can be translated to larger studies examining the potential combined effect of energy availability and genotype.

The proposed pilot study is a single-site, 2-period study in healthy female volunteers. The study will enroll approximately 300 participants with a target for study completion of 36 subjects. Eligible participants will be females >= 18 years of age. Eligible participants will have had menarche at or before 14 years of age and no earlier than age 11. Eligible participants will have a gynecological age (years after menarche) of 14 years or less. The upper age limit will vary based on each subject s age of menarche and fall between 25 and 28 among participants. Eligible participants will confirm at the pre-screening call having normal menstrual cycles (self-reported) for at least the previous 2 months and ovulation will be confirmed during the menstrual cycle before the start of intervention.

The primary outcome will be changes in daytime LH pulse frequency, when comparing NEA vs DEA. Secondary measures will evaluate past reproductive history, family history, and current metabolic status using medical history interviews, lifestyle questionnaires and maximum oxygen uptake (as a measure of fitness). Resting energy expenditure, body composition as well as metabolic and stress hormones will be measured at baseline and in association with the interventions.

• INCLUSION CRITERIA:

Participants will be enrolled with distribution across race. Each potential participant must meet all of the following inclusion criteria in order to be eligible to enroll in the study:

1. Female
2. Between 18 and 28 years of age (inclusive)
3. Reported menarche between the ages of 11 and 14 years
4. Gynecological age of <= 14 years
5. A history of self-reported regular menstrual cycles when not on contraceptive medication of between 25 and 35 days (inclusive) at prescreen and knowledge of date of onset of menses before the screening visit
6. A BMI of 18.5 to 27 kg x m^2 and a weight >= 93 lbs.
7. Agrees to use barrier contraception method for the duration of the study and the follow up period
8. Agrees to abstain from alcohol consumption during both 5-day diet/exercise study interventions
9. Agrees to abstain from donating blood during the study and within 30 days of completing the study
10. Agrees to abstain from biotin supplements for the duration of the study
11. Is willing and able to fulfill the requirements of the protocol and to provide informed consent
12. Able to speak and read English
13. Lives within 50 miles of the Clinical Research Unit

EXCLUSION CRITERIA:

A potential participant meeting any of the following exclusion criteria is not eligible to enroll in the study:

1. Currently lactating or pregnant or planning on becoming pregnant for the duration of the study

2. Has ever given birth

3. History in the past 3 months of dieting or weight loss amounting to greater than 2 kg

4. > 4 hours per week of aerobic exercise for the past 3 months

5. Has initiated training for an athletic sport or event in the past 3 months that, in the opinion of the investigator, may interfere with the results of the study

6. Currently using hormone-based contraception, including those administered orally, vaginally, via injection, sub-dermally, or transdermally

7. Current use of medications or supplements that may interfere with the results of the study, including:

   i.Steroids

   ii.Hormone-based contraception

   iii.Sleeping pills

   iv.Homeopathic substances (e.g. Chinese herbs, protein or other powders, and other-the-counter extracts)

   v.Stimulants (e.g. Ritalin)

   vi.Antidepressants or anti-epileptic medications or centrally acting anti-hypertensive medications

8. Current use of recreational drugs (alcohol intake will be monitored and excluded during the two intervention periods)

9. Unable to consume food containing dairy or nuts

10. Has regular overnight awake periods, for example overnight or rotating shift work, that will continue during the study, at the discretion of the investigator

11. Has currently or has a history of any of the following: autoimmune, heart, liver, renal disease, diabetes, or another health condition deemed by the PI to be a contraindication to study participation.

Additional Eligibility Criteria to be Met Prior to Start of Intervention(s):

Criteria 1 Habitual energy intake between 35-55 kcal/kg LBM*day

Criteria 2 VO2max <= 40 ml/kg/min with the option to increase this at the discretion of the PI, depending on the current and past exercise level of the participant.

Criteria 3 Hemoglobin, prolactin and TSH within normal female range for testing laboratory.

Criteria 4 Ovulation confirmed in the cycle before each study intervention by self-reported positive urine test, ultrasound and/or progesterone blood-levels

Aggregated and summarized data will be made available as part of associated publication.@@@Deidentified patient-level data, with appropriate IRB approvals, could be made available for sharing.@@@@@@This project generates data from a behavioral intervention study that includes the following data types:@@@-Demographic data@@@-Clinical evaluation@@@-Patient questionnaires@@@-Laboratory studies (including hormonal testing)@@@-Metabolic paramaters (e.g., VO2 max, REE)@@@-Imaging studies (e.g., Ultrasound, DEXA)@@@

Data will be made available as part of published manuscripts to individual researchers upon reasonable request related to the original study protocol research questions, after the study team has finished its analysis.

Aggregated summary and statistical analysis will be publicly available. All the patient-level data will require controlled access.@@@@@@@@@@@@Outside investigators may apply for research collaborations related to the original research questions, that will be IRB approved for use and a data transfer agreement will be signed, and coded, deidentified data will be provided with a data dictionary. For studies unrelated to the original research, only deidentified data can be provided and only when subjects consented to future research.