Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
This study is performed to analyze various aspects of biofilms on ureteral stents.
The study is performed to provide a better understanding of the influence of biofilms on ureteral stents on the entire spectrum of stent-associated morbidity.
Moreover, it could provide more detailed knowledge about the correlation of the occurrence of biofilms on ureteral stents and findings in urinary analysis, which could serve as a decision support whether patients with ureteral stents undergoing further interventions (for example ESWL or simple secondary URS) should receive antibiotic prophylaxis.
Correlation of results of urinary bacteriologies (assessed pre- and intraoperatively) and biofilm analysis on stents could provide information if "targeted antibiotic treatment" based on urinary bacteriology only is possible at all.
Correlation of urinary analysis and conventional urinary bacteriology could provide a valuable contribution regarding the prediction of bacteriuria and the necessity of postponing operations.
Additionally, the study is intended to provide an overview over the recent bacterial species that are involved into biofilms on ureteral stents and their resistance to antibiotics. This could provide valuable information about the choice of antibiotics in cases were treatment has to be performed empirically.
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Patients with temporary ureteral stent | Assessment of patients transiently undergoing ureteral stenting. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Stent examination, urinary analysis, USSQ questionnaire | Other |
|
| Measure | Description | Time Frame |
|---|---|---|
| Total biofilm mass on ureteral stents. | Time of stent removal: Four weeks (range 2-6 weeks) after stent insertion | |
| Assessment of morbidity using the Ureteral Stent Symptom Questionnaire (USSQ) | Day before stent removal (i.e. 4 weeks (range 2-6 weeks) after insertion) |
| Measure | Description | Time Frame |
|---|---|---|
| Assessment of morbidity using the Ureteral Stent Symptom Questionnaire (USSQ) | One week after stent insertion; Four weeks (range 2-6 weeks) after stent removal. | |
| Urinary analysis (pH, nitrite, leucocyturia, hematuria). | Day before stent removal (i.e. 4 weeks (range 2-6 weeks) after insertion) |
Not provided
Inclusion Criteria:
Exclusion Criteria:
Not provided
Not provided
Not provided
Patients undergoing ureteral stenting for the preparation of a secondary intervention in an inpatient setting at the department of urology of KSSG.
Not provided
| Name | Affiliation | Role |
|---|---|---|
| Dominik Abt, Dr. med. | Department of Urology | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Urological Department, Cantonal Hospital of St. Gallen | Sankt Gallen | Canton of St. Gallen | 9007 | Switzerland |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 30981280 | Derived | Buhmann MT, Abt D, Nolte O, Neu TR, Strempel S, Albrich WC, Betschart P, Zumstein V, Neels A, Maniura-Weber K, Ren Q. Encrustations on ureteral stents from patients without urinary tract infection reveal distinct urotypes and a low bacterial load. Microbiome. 2019 Apr 13;7(1):60. doi: 10.1186/s40168-019-0674-x. |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Urine Culture | Before study inclusion; Day before stent removal (i.e. 4 weeks (range 2-6 weeks) after insertion); Intraoperatively (during stent removal) |
| Rapid immunoassay test for bacteriuria | Day before stent removal (i.e. 4 weeks (range 2-6 weeks) after insertion) |
| Next generation sequencing of biofilms | To identify bacterial species that are involved into biofilms on ureteral stents. | Day of stent removal: Four weeks (range 2-6 weeks) after stent insertion |
| Number of bacteria on stent surface (cell count as estimated by scanning electron microscopy) | Day of stent removal: Four weeks (range 2-6 weeks) after stent insertion |
| Number of bacteria on stent surface (determined via quantitative PCR) | Day of stent removal: Four weeks (range 2-6 weeks) after stent insertion |