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| ID | Type | Description | Link |
|---|---|---|---|
| Local Grant 2009 | Other Grant/Funding Number | 0916302 | |
| 2010-A00082-37 | Other Identifier | French ANSM |
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The objective is to compare women's platelet answer in the last month of pregnancy to women old enough to procreate, no pregnancy and without hormonal treatment.
The hypothesis of research is that the decrease of platelet answer in pregnant women could not only limit the occurence of embolic accidents but also explain the lowest incidence of the arterial thrombosis in comparison with the venous thrombosis.
This is a monocentric study comparing a group of pregnant women and a group of "no pregnancy" women.
The goal of the present study is to determine the impact of pregnancy on platelet function. For this, the study propose to explore, in women in the last month of pregnancy, the ability of platelets to aggregate after activation by strong agonists but also their ability to adhere to a matrix and form thrombies.
The study will include 30 pregnant and 30 "no pregnancy" control women, of childbearing age and not taking hormone therapy. From a blood sample, two types of examination will be implemented:
A) In whole blood, we will evaluate the percentage of platelet adhesion to collagen matrix or fibrinogen and the volume of thrombies formed.
B) In washed platelets and whole blood, we shall determine the percentage of aggregation after activation by different agonists and the amount of certain membrane markers of platelet activation. Furthermore, an assay of estradiol plasma will be made.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Pregnancy | Experimental | Blood sample |
|
| No pregnancy | Experimental | Blood sample |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Blood sample | Other | In washed platelets and whole blood, study shall determine the percentage of aggregation after activation by different agonists and the amount of certain membrane markers of platelet activation. Furthermore, an assay of estradiol plasma will be made. In whole blood, study will evaluate the percentage of platelet adhesion to collagen matrix or fibrinogen and the volume of thrombies formed. |
| Measure | Description | Time Frame |
|---|---|---|
| surface covered by platelets | Percentage of surface covered by platelets (using the software Metamorph) | baseline |
| Volume of thrombies | Total volume of thrombies in μm3 (using the software Metamorph) | baseline |
| Measure | Description | Time Frame |
|---|---|---|
| Platelet aggregation | Percentage of platelet aggregation (whole blood and washed platelets) | baseline |
| chemokine Platelet Factor 4 | Quantity of the chemokine Platelet Factor 4 (PF4) (IU/ml) secreted by the granules after activation by these agonists (washed platelets) |
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Inclusion Criteria:
Signature of the form of consent
Absence of any physical or psychic pathology which can interfere with the normal realization of the study
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| PARANT Olivier, MD PhD | University Hospital, Toulouse | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University Hospital of Toulouse | Toulouse | France |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 26435170 | Result | Valera MC, Parant O, Cenac C, Arnaud C, Gallini A, Hamdi S, Sie P, Arnal JF, Payrastre B. Platelet Adhesion and Thrombus Formation in Whole Blood at Arterial Shear Rate at the End of Pregnancy. Am J Reprod Immunol. 2015 Dec;74(6):533-41. doi: 10.1111/aji.12433. Epub 2015 Oct 4. |
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| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| D011247 | Pregnancy |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
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|
| baseline |
| Thromboglobulin | Quantity of thromboglobulin (in ng / ml) secreted by the granules after activation by these agonists (washed platelets) | baseline |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |
| D012098 | Reproduction |
| D055703 | Reproductive Physiological Phenomena |
| D012101 | Reproductive and Urinary Physiological Phenomena |