Technical Optimization of Detection of KRAS, BRAF and NRAS Mutations on Tumor DNA Circulating in Metastatic Colorectal Cancer

Prospective Study of Technical Optimization of the Detection of KRAS Mutations, BRAF and NRAS by Next Generation Sequencing on Tumor DNA Circulating in Metastatic Colorectal Cancer.

The objective of this study is to optimize the search by next-generation sequencing (NGS) mutations in the KRAS, BRAF and NRAS on circulating tumor DNA and compare the genetic profiles obtained with those from tumors embedded in paraffin

Inclusion Criteria:

• Patients with metastatic or locally advanced colorectal carcinoma with a KRAS, BRAF or NRAS mutation
• Age ≥ 18 years
• Patient information and written informed consent form signed
• Patient must be affiliated to a social security system

Exclusion Criteria:

• Patients whose health-cons indicates a blood sample 10mL
• Age < 18 years
• Pregnant or breast feeding females
• Persons deprived of liberty or under supervision