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Recent epidemiological studies in France showed a high prevalence of obesity (14.5%) and its strong increase in the last 20 years. Among the many complications associated with obesity, liver complications (steatosis and steatohepatitis [NASH]) are among the most common. Semaphorins were described in the early 1990. More than 20 types of these proteins have been reported to date. These proteins were used for neural development. Since many functions have also been described. The semaphorins are involved in numerous physiological or physiopathological processes (cardiac morphogenesis, vascular growth, tumor progression), the regulation of immune cells and liver fibrosis. Preliminary studies have allowed to show that dendritic cells infiltrate adipose tissue and initiate the activation of T cells and inflammation. Immune semaphorin are new players in the regulation of inflammation and immune reactions.
The role of immune semaphorin in regulating inflammation in the two compartments (liver and adipose tissue) could be a crucial step that could lead to more severe liver damage. Its dysregulation could explain NASH injuries. The goal is to identify a new mode of regulation of cellular homeostasis in the fatty liver disease. These factors may serve as diagnostic markers or future therapeutic targets.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Morbid obese patients | Other |
| |
| Control patients | Other |
| |
| Patients with overweight, steatosis and steatohepatitis | Other |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Evaluation of the staging of fibrosis liver | Other | The investigators will determine the severity of steatosis, inflammation and fibrosis by histological study of liver biopsies |
|
| Measure | Description | Time Frame |
|---|---|---|
| Evaluation of the staging of liver fibrosis | The investigators determine the stage of liver fibrosis by histological study of biopsies | Day 0 |
| Determination of the expression of level of semaphorin | The investigators determine by technic of Reverse Transcription Polymerase Chain Reaction (RT-PCR) the expression of level of semaphorin | Day 0 |
| Determination of the composition of immunity cells | The investigators determine the composition of immunity cells by Immunohistochemical and biochemical analyses (Western Blotting) | Day 0 |
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Morbid obese patients
Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Albert TRAN, MDPhD | Centre Hospitalier Universitaire de Nice | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Service d'Hépato-Gastroentérologie - Hôpital de l'Archet | Nice | 06003 | France |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 28323124 | Result | Patouraux S, Rousseau D, Bonnafous S, Lebeaupin C, Luci C, Canivet CM, Schneck AS, Bertola A, Saint-Paul MC, Iannelli A, Gugenheim J, Anty R, Tran A, Bailly-Maitre B, Gual P. CD44 is a key player in non-alcoholic steatohepatitis. J Hepatol. 2017 Aug;67(2):328-338. doi: 10.1016/j.jhep.2017.03.003. Epub 2017 Mar 16. | |
| 28550439 | Derived |
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| Determine the expression level of semaphorin | Other | The investigators will determine the expression level (gene and protein) of immune semaphorin and their (s) receptor (s) in the liver and subcutaneous and visceral adipose tissue by RT-PCR, |
|
| Determination of the composition of immunity cells | Other | The investigators determine the composition of immunity cells by immunohistochemical and biochemical analyses (Western Blotting ) |
|
| Sans A, Bailly L, Anty R, Sielezenef I, Gugenheim J, Tran A, Gual P, Iannelli A. Baseline Anthropometric and Metabolic Parameters Correlate with Weight Loss in Women 1-Year After Laparoscopic Roux-En-Y Gastric Bypass. Obes Surg. 2017 Nov;27(11):2940-2949. doi: 10.1007/s11695-017-2720-8. |
| ID | Term |
|---|---|
| D065626 | Non-alcoholic Fatty Liver Disease |
| ID | Term |
|---|---|
| D005234 | Fatty Liver |
| D008107 | Liver Diseases |
| D004066 | Digestive System Diseases |
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