Pollen allergen extracts are prepared locally and checked for efficacy by comparison to imported allergens and specific IgE.

Skin prick test (SPT) and specific IgE (sIgE) are approved for evaluation of allergen sensitization. Local allergen extracts are cheaper and more available but need standardization. Pollen allergen extracts: Bermuda, Johnson and careless weed are locally prepared and compared mean wheal diameter of skin prick test to imported allergens and also to specific IgE.

Inclusion Criteria:

• Positive skin prick test to Bermuda or Johnson or careless weed

Exclusion Criteria:

• Chronic diseases such as heart disease, liver, renal and severe sepsis
• Severe allergic symptoms such as anaphylaxis
• Extensive skin rashes
• Pregnancy

People 18-60 years of age who recently have positive skin prick test to grass pollen or careless weed are pricked with local and imported Bermuda grass, Johnson grass and careless weed allergen extracts. Specific IgE levels are also measured at the same day.