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The main objective of this research is to study the links between changes in the intestinal microbiota (in terms of diversity) during the first 6 weeks of life for preterm infants and the presence / absence of a TH1 immune status at 36 months of age.
The secondary objectives are to study the links between changes of the intestinal microbiota premature infants in terms of:
-AND-
The links between certain variables known in the literature and neonatal microbiota will be confirmed / studied in our population:
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| The study population | The study population corresponds to infants born at less than 33 weeks of gestation. |
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| Measure | Description | Time Frame |
|---|---|---|
| The presence/absence of a Th1 type immune orientation | Immune orientation will be determined according to the following ratio determined by lymphocyte stimulation tests: INF-gamma/(INF-gamma+IL+4). The latter ratio varies between 0 and 0.5 with IL-4 > INF-gamma (TH2 orientation) and between 0.5 and 1 when INF-gamma > IL-4 (TH1 orientation). | 36 months |
| Measure | Description | Time Frame |
|---|---|---|
| Blood lymphocyte subset determination | Subsets are determined according to the presence/absence of the following differentiation clusters: CD3, CD4, CD8, CD19, CD25, CD56, CD127, FOXP3. | 36 months |
| Measure | Description | Time Frame |
|---|---|---|
| Serum Immunoglobulin A level | 36 months | |
| Serum Immunoglobulin G level | 36 months | |
| Serum Immunoglobulin M level |
Inclusion Criteria:
Exclusion Criteria:
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The study population consists of premature babies born at < 33 months gestation.
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| Name | Affiliation | Role |
|---|---|---|
| Anne Filleron, MD, PhD | Centre Hospitalier Universitaire de Nīmes | Study Director |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| CHRU de Montpellier - Hôpital Arnaud de Villeneuve | Montpellier | 34295 | France | |||
| CHRU de Nîmes - Hôpital Universitaire Carémeau |
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| ID | Term |
|---|---|
| D047928 | Premature Birth |
| ID | Term |
|---|---|
| D007752 | Obstetric Labor, Premature |
| D007744 | Obstetric Labor Complications |
| D011248 | Pregnancy Complications |
| D005261 | Female Urogenital Diseases and Pregnancy Complications |
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Blood samples will be drawn at 36 months for lymphocyte subpopulation characterization, stimulation tests and biobanking. Fecal samples at indicated time points will also be banked (bacterial rDNA will be extracted).
| 36 months |
| Serum Immunoglobulin E level | 36 months |
| The number of days in good health and no medications | 36 months |
| Richness of fecal microbiota (number of operational taxonomic units observed) | Day 0 (first meconium) |
| Richness of fecal microbiota (number of operational taxonomic units observed) | Week 1 |
| Richness of fecal microbiota (number of operational taxonomic units observed) | Week 2 |
| Richness of fecal microbiota (number of operational taxonomic units observed) | Week 3 |
| Richness of fecal microbiota (number of operational taxonomic units observed) | Week 4 |
| Richness of fecal microbiota (number of operational taxonomic units observed) | Week 5 |
| Richness of fecal microbiota (number of operational taxonomic units observed) | Week 6 |
| Richness of fecal microbiota (number of operational taxonomic units observed) | 12 months |
| Richness of fecal microbiota (number of operational taxonomic units observed) | 24 months |
| Richness of fecal microbiota (number of operational taxonomic units observed) | 36 months |
| Diversity of fecal microbiota (Shannon's index) | Day 0 (first meconium) |
| Diversity of fecal microbiota (Shannon's index) | Week 1 |
| Diversity of fecal microbiota (Shannon's index) | Week 2 |
| Diversity of fecal microbiota (Shannon's index) | Week 3 |
| Diversity of fecal microbiota (Shannon's index) | Week 4 |
| Diversity of fecal microbiota (Shannon's index) | Week 5 |
| Diversity of fecal microbiota (Shannon's index) | Week 6 |
| Diversity of fecal microbiota (Shannon's index) | 12 months |
| Diversity of fecal microbiota (Shannon's index) | 24 months |
| Diversity of fecal microbiota (Shannon's index) | 36 months |
| Functional richness of fecal microbiota (number of functional groups observed) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Day 0 (first meconium) |
| Functional richness of fecal microbiota (number of functional groups observed) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Week 1 |
| Functional richness of fecal microbiota (number of functional groups observed) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Week 2 |
| Functional richness of fecal microbiota (number of functional groups observed) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Week 3 |
| Functional richness of fecal microbiota (number of functional groups observed) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Week 4 |
| Functional richness of fecal microbiota (number of functional groups observed) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Week 5 |
| Functional richness of fecal microbiota (number of functional groups observed) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Week 6 |
| Functional richness of fecal microbiota (number of functional groups observed) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | 12 months |
| Functional richness of fecal microbiota (number of functional groups observed) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | 24 months |
| Functional richness of fecal microbiota (number of functional groups observed) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | 36 months |
| Functional diversity of fecal microbiota (Shannon's index) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Day 0 (first meconium) |
| Functional diversity of fecal microbiota (Shannon's index) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Week 1 |
| Functional diversity of fecal microbiota (Shannon's index) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Week 2 |
| Functional diversity of fecal microbiota (Shannon's index) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Week 3 |
| Functional diversity of fecal microbiota (Shannon's index) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Week 4 |
| Functional diversity of fecal microbiota (Shannon's index) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Week 5 |
| Functional diversity of fecal microbiota (Shannon's index) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | Week 6 |
| Functional diversity of fecal microbiota (Shannon's index) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | 12 months |
| Functional diversity of fecal microbiota (Shannon's index) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | 24 months |
| Functional diversity of fecal microbiota (Shannon's index) | Functional groups are determined via the classification of each OTU according to the following factors: 1) pathogenicity (pathogen/intermediate/commensal); 2) aerobic versus anaerobic; 3) Cocci + versus others. | 36 months |
| The relative abundance of primary fecal microbiota families | Day 0 (first meconium) |
| The relative abundance of primary fecal microbiota families | Week 1 |
| The relative abundance of primary fecal microbiota families | Week 2 |
| The relative abundance of primary fecal microbiota families | Week 3 |
| The relative abundance of primary fecal microbiota families | Week 4 |
| The relative abundance of primary fecal microbiota families | Week 5 |
| The relative abundance of primary fecal microbiota families | Week 6 |
| The relative abundance of primary fecal microbiota families | 12 months |
| The relative abundance of primary fecal microbiota families | 24 months |
| The relative abundance of primary fecal microbiota families | 36 months |
| Fecal microbiota unifrac ordination score | Day 0 (first meconium) |
| Fecal microbiota unifrac ordination score | Week 1 |
| Fecal microbiota unifrac ordination score | Week 2 |
| Fecal microbiota unifrac ordination score | Week 3 |
| Fecal microbiota unifrac ordination score | Week 4 |
| Fecal microbiota unifrac ordination score | Week 5 |
| Fecal microbiota unifrac ordination score | Week 6 |
| Fecal microbiota unifrac ordination score | 12 months |
| Fecal microbiota unifrac ordination score | 24 months |
| Fecal microbiota unifrac ordination score | 36 months |
| Nîmes |
| 30029 |
| France |
| Réseau GRANDIR EN LANGUEDOC-ROUSILLON | Saint-Gély-du-Fesc | 34980 | France |
| D000091642 | Urogenital Diseases |