The aim of this study is to establish a platform of detecting and sorting circulating tumor stem cells from peripheral blood in HCC patients; to investigate the relationship between circulating tumor stem cells and their effects on postoperative recurrence and metastasis, in order to provide a new therapeutic target for hepatocellular carcinoma treatment.

Inclusion Criteria:

1. Male or female patients > 18 years and <=70 years of age;
2. No dysfunction in major organs; Blood routine, kidney function, cardiac function and lung function are basically normal.
3. Patients who can understand this trial and have signed information consent

Exclusion Criteria:

1. Reject to attend；
2. Impossible to come to our hospital for physical examination regularly;
3. Patients with other diseases which may affect the treatment mentioned

250 healthy volunteers, 250 patients with hepatitis cirrhosis, 500 patients with HCC;