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The purpose of this study is to investigate for the broad immunological effects of administering measles vaccine (MV) and diphtheria-tetanus-whole cell pertussis vaccine (DTP) to 9 month old Gambian infants, either alone or together. Effects on vaccine-specific immune responses, innate immunity, and immune memory were studied. The hypothesis is that when MV and DTP are given together there will be more inflammation and this will interfere with generation of immunity to the vaccine and to other non-vaccine related stimuli.
Many studies demonstrate that routine Expanded Program on Immunisation (EPI) vaccinations have "non-specific" or "heterologous" effects on child survival that cannot be accounted for by protection against the vaccine specific disease. These effects were overlooked in the past because it was assumed that the impact on survival would be proportional to reduction in the targeted infection. Large epidemiological studies in Bangladesh and Guinea-Bissau have shown that live vaccines, including measles vaccine (MV), have beneficial effects on overall mortality which cannot be explained by protection against the vaccine specific disease. Deleterious non-specific effects were shown convincingly in the high-titre MV trials in which girls, but not boys, had a two-fold increase in mortality even though the vaccine was fully protective against measles. A reinterpretation of these trials indicated that the most likely explanation was that the increased female mortality was due to inactivated vaccines provided after MV. It has further been shown in smaller observational studies that receiving diphtheria, tetanus and whole cell pertussis combined vaccine (DTwP) either with or after MV negates the beneficial effects of MV, and may lead to increased mortality. However, the increased mortality of girls in male-female twin pairs was not apparent when MV was given after the last dose of DTwP. In the present EPI schedule in The Gambia, DTwP is administered as a single dose vaccine at 2, 3 and 4 months, and MV at nine months. However, many children, particularly in low income countries, present late for their vaccines and may subsequently receive them in the wrong order. In particular DTP is often given at the wrong time point, e.g. the third DTP dose (DTP3) with MV. Given the increasing evidence that the order in which vaccines are administered is crucial, the routine practice of administering missed EPI vaccines at late presentation or boosting with DTP in year 2 may not be advisable.
The mechanism behind these effects is unknown, and whether there is an immunological basis has yet to be explored. The majority of controlled trials of vaccine efficacy have focused on vaccine specific antibody (Ab) responses to test vaccine efficacy, and few studies have investigated T cell memory induction, despite the fact that it is likely to be critical for long term protection. Indeed, the cellular response to MV seems to be crucial in protecting against severe disease and death, and cellular responses are required to protect against Bordetella pertussis infection. Live vaccines such as bacillus Calmette-Guerin (BCG) and MV have been shown to stimulate Th1 type immune responses. The immune response to DTwP, a killed vaccine, tends to be Th2 biased in early life, despite the fact that the whole cell pertussis component biases towards a Th1 response. DTwP contains an aluminium based adjuvant, aluminium hydroxide, which is excellent at promoting humoral and Th2 responses, but poor at generating good cell mediated immunity and long term T cell memory. This polarisation of cellular reactivity to live and killed vaccines may explain why administering a killed vaccine with a live vaccine abrogates the beneficial effect of the live vaccine. Conversely, administering a live vaccine after DTwP may cause a shift towards protective Th1 type immunity, thus diminishing the harmful effects of DTwP and providing a non-specific survival benefit. Interestingly, aluminium hydroxide has been shown to cause enhanced susceptibility to tuberculosis (TB) in animal models. Thus the aluminium adjuvant in DTwP may play a role in the deleterious effect of DTwP on live vaccines, possibly through an influence on the generation of T cell memory to other infections and vaccines.
Attributing the observed effects of the DTwP / MV interactions entirely to shifts in the T helper cell 1 (Th1) / Th2 profile is likely to be an over simplification, but provides a good starting point for unravelling this phenomenon. Other arms of the immune response that need to be considered are regulatory T cells (Tregs) and the Th17 inflammatory T cell lineage. The former are a heterogeneous group of naturally occurring and induced T cells, and have been shown to play a regulatory role in immunity to a number of infectious diseases, but almost nothing is known about their generation or functional role in vaccine immunogenicity in humans. Newborns are known to have high levels of functional Tregs, and these are likely to be essential in controlling the immune response to antigens encountered in early life, as well as modulating self reactive responses. Measles vaccination causes suppression of T cell responses, and measles vaccinated children upregulate Forkhead Box P3 (FOXP3) expression (Ota et al, personal communication), suggesting a role for Tregs. Thus Treg induction is likely to be an important component of the immune response to MV.
This study will investigate in detail the immunological consequences of giving DTP or MV alone or at the same time. The study will involve an intervention trial to analyse the effect of giving DTP with MV on the generation of T cell memory (effector and central), humoral responses, pro-inflammatory cytokine profile (Th1, Th2, Th17) and Treg responses to measles and recall antigens. Our detailed immunological studies will provide vital immunological data on potential mechanisms of the DTP / MV interaction. A comprehensive understanding of the immunological effects of administering either DTP alone or DTP and MV simultaneously will help us understand why these interventions might be detrimental, and may suggest the need to refine current EPI practices. It is also crucial to gain a detailed understanding of the immunological effects of possible deleterious interactions between the current EPI vaccines, before introducing new vaccines that are becoming available for testing in early life e.g. TB and malaria vaccines. Furthermore, understanding mechanisms whereby MV provides survival benefits might identify strategies that can be harnessed in future vaccine design.
All infants will be given a MV challenge at 18 months of age and immunological parameters will be analysed 1 month later. This will establish whether any vaccine group effects at 10 months off age persist until 18 months of age and influence the immune response to a new immune challenge.
Two primary hypotheses as detailed below will be tested as a starting point, but other immunological assays will be conducted. Furthermore all analyses will take sex into account since the heterologous effects of vaccines are different in males and females, with females generally being more susceptible.
Hypotheses:
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| MV at 9 months | Active Comparator | Measles Vaccine at 9 months of age. Measles Vaccine at 18 months of age. All vaccines given as per normal Gambia schedule until 9 months of age, including third dose of diphtheria-tetanus-whole cell pertussis (DTP3), hepatitis B vaccine (HBV) and oral polio vaccine (OPV) at four months of age. At 9 months of age given a single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid. Yellow fever vaccine (YF) and OPV administered at 11 months of age. Given a standard MV challenge at 18 months of age. |
|
| DTP + MV at 9 months | Active Comparator | Measles Vaccine at 9 months of age. DTP Vaccine at 9 months of age. Measles Vaccine at 18 months of age. DTP3 dose withheld and given HBV and OPV at four months of age. At 9 months of age given a single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid, and i.m. DTP (Serum Institute of India Ltd.) in the thigh. Yellow fever vaccine (YF) and OPV administered at 11 months of age. Given a standard MV challenge at 18 months of age. |
|
| DTP at 9 months | Active Comparator | DTP Vaccine at 9 months of age. Measles Vaccine at 18 months of age. DTP3 dose withheld and given HBV and OPV at four months of age. At 9 months of age given i.m. DTP (Serum Institute of India Ltd.) in the thigh. MV, OPV and YF administered at 11 months of age. Given a standard MV challenge at 18 months of age. |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Measles Vaccine at 9 months of age | Biological | Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid at 9 months of age |
| Measure | Description | Time Frame |
|---|---|---|
| Effect on Interferon (IFN)-Gamma : Interleukin (IL)-4 Ratio in Plasma | The IFN-gamma:IL-4 cytokine ratio in plasma samples taken 4 weeks after vaccination measured by multiplex assay using the Bio-Plex 200 Suspension Array system. Ratio compared between the 3 intervention groups with the hypothesis that the MV+DTP group will have a higher ratio than the other 2 groups. | 4 weeks after vaccination |
| Effect on CD4+FOXP3+CD127- Regulatory T Cell Frequencies | Regulatory T cell (Treg) frequencies (among all gated CD4+ T cells) at 9 and 10 months of age determined by flow cytometry and compared between the 3 intervention groups with the primary hypothesis that the MV+DTP group will have lower Treg frequencies than the MV group. | 4 weeks (9 months and 10 months of age) |
| Measure | Description | Time Frame |
|---|---|---|
| Effect of Vaccine Group on Measles Antibody Levels | Effect of combining MV and DTP on measles antibody responses to see if combining vaccines interferes with antibody generation. Plasma measles antibodies measured by haemagglutination inhibition assay at 9 and 10 months of age. | 4 weeks (9 and 10 months of age) |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Katie L Flanagan, PhD | University of Tasmania, Australia | Principal Investigator |
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| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 12842371 | Background | Aaby P, Jensen H, Samb B, Cisse B, Sodemann M, Jakobsen M, Poulsen A, Rodrigues A, Lisse IM, Simondon F, Whittle H. Differences in female-male mortality after high-titre measles vaccine and association with subsequent vaccination with diphtheria-tetanus-pertussis and inactivated poliovirus: reanalysis of West African studies. Lancet. 2003 Jun 28;361(9376):2183-8. doi: 10.1016/S0140-6736(03)13771-3. | |
| 11110734 |
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The data will be made available upon written request
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| ID | Title | Description |
|---|---|---|
| FG000 | MV at 9 Months | All vaccines given as per normal Gambia schedule until 9 months of age, including third dose of diphtheria-tetanus-whole cell pertussis (DTP3), hepatitis B vaccine (HBV) and oral polio vaccine (OPV) at four months of age. At 9 months of age given a single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid. Yellow fever vaccine (YF) and OPV administered at 11 months of age. Given a standard MV challenge at 18 months of age. Measles Vaccine at 9 months of age: Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid at 9 months of age Measles Vaccine at 18 months of age: Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid at 18 months of age |
| FG001 | DTP + MV at 9 Months | DTP3 dose withheld and given HBV and OPV at four months of age. At 9 months of age given a single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid, and i.m. DTP (Serum Institute of India Ltd.) in the thigh. Yellow fever vaccine (YF) and OPV administered at 11 months of age. Given a standard MV challenge at 18 months old. Measles Vaccine at 9 months of age: Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid at 9 months of age Diphtheria-tetanus-whole cell pertussis vaccine at 9 months of age: Single intramuscular (i.m.) dose of DTP (Serum Institute of India Ltd.) into the thigh at 9 months of age Measles Vaccine at 18 months of age: Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the de |
| FG002 | DTP at 9 Months | DTP3 dose withheld and given HBV and OPV at four months of age. At 9 months of age given i.m. DTP (Serum Institute of India Ltd.) in the thigh. MV, OPV and YF administered at 11 months of age. Given a standard MV challenge at 18 months of age. Diphtheria-tetanus-whole cell pertussis vaccine at 9 months of age: Single intramuscular (i.m.) dose of DTP (Serum Institute of India Ltd.) into the thigh at 9 months of age Measles Vaccine at 18 months of age: Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid at 18 months of age |
| Title | Milestones | Reasons Not Completed | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Overall Study |
|
|
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| ID | Title | Description |
|---|---|---|
| BG000 | MV at 9 Months | All vaccines given as per normal Gambia schedule until 9 months of age, including third dose of diphtheria-tetanus-whole cell pertussis (DTP3), hepatitis B vaccine (HBV) and oral polio vaccine (OPV) at four months of age. At 9 months of age given a single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid. Yellow fever vaccine (YF) and OPV administered at 11 months of age. Given a standard MV challenge at 18 months of age. Measles Vaccine at 9 months of age: Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid at 9 months of age Measles Vaccine at 18 months of age: Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid at 18 months of age |
| Units | Counts |
|---|---|
| Participants |
|
| Title | Description | Population Description | Parameter Type | Dispersion Type | Unit of Measure | Calculate Percentage | Denominator Units Selected | Denominators | Classes |
|---|---|---|---|---|---|---|---|---|---|
| Age, Continuous | Mean |
| Type | Title | Description | Population Description | Reporting Status | Anticipated Posting Date | Parameter Type | Dispersion Type | Unit of Measure | Calculate Percentage | Time Frame | Units Analyzed | Denominator Units Selected | Arm/Group Information | Denominators | Classes | Analyses | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Primary | Effect on Interferon (IFN)-Gamma : Interleukin (IL)-4 Ratio in Plasma | The IFN-gamma:IL-4 cytokine ratio in plasma samples taken 4 weeks after vaccination measured by multiplex assay using the Bio-Plex 200 Suspension Array system. Ratio compared between the 3 intervention groups with the hypothesis that the MV+DTP group will have a higher ratio than the other 2 groups. | Not all participants had this assay done due to the follow reasons: failure to attend the appointment, unwell on the day of follow up, dropped out of the study, assay failure, insufficient blood sample volume or quality. | Posted | Mean | Standard Deviation | ratio of cytokines in pg/mL | 4 weeks after vaccination |
|
10 months (from 9 months of age to 19 months of age)
Infants were followed up monthly for 10 months and a clinical questionnaire was completed at each visit.
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| ID | Title | Description | Deaths (Affected) | Deaths (At Risk) | Serious Events (Affected) | Serious Events (At Risk) | Other Events (Affected) | Other Events (At Risk) |
|---|---|---|---|---|---|---|---|---|
| EG000 | MV Vaccination at 9 Months | Infants administered MV at 9 months of age | 1 |
| Term | Organ System | Source Vocabulary | Assessment Type | Notes | Statistical Information |
|---|---|---|---|---|---|
| Hospital admission with fever and rash | Infections and infestations | Systematic Assessment |
| Term | Organ System | Source Vocabulary | Assessment Type | Notes | Statistical Information |
|---|---|---|---|---|---|
| Fever | Infections and infestations | Systematic Assessment | Fever of unknown cause |
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| Title | Organization | Phone | Extension | |
|---|---|---|---|---|
| Katie Flanagan | University of Tasmania | +61 3 6777 4081 | katie.flanagan@ths.tas.gov.au |
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| ID | Term |
|---|---|
| D000375 | Aging |
| ID | Term |
|---|---|
| D048788 | Growth and Development |
| D010829 | Physiological Phenomena |
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|
| DTP Vaccine at 9 months of age | Biological | Single intramuscular (i.m.) dose of diphtheria-tetanus-whole cell pertussis vaccine (Serum Institute of India Ltd.) into the thigh at 9 months of age |
|
|
| Measles Vaccine at 18 months of age | Biological | Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid at 18 months of age |
|
|
| Effect of Vaccine Group on Effector Memory T Cells (TEM) |
CD4+CD45RO+CD62L- effector memory T cells measured by flow cytometry at 9 and 10 months of age |
| 4 weeks (9 and 10 months of age) |
| Effect of Vaccine Group on Diphtheria and Tetanus Antibody Levels | Plasma diphtheria and tetanus toxoid antibodies were measured by multiplex microsphere-based fluorescent immunoassay at 9 and 10 months of age. | 4 weeks (9 and 10 months of age) |
| Effect of Vaccine Group on Pertussis Toxoid Antibody Levels | Plasma pertussis toxoid antibodies were measured by multiplex microsphere-based fluorescent immunoassay at 9 and 10 months of age | 4 weeks (9 and 10 months of age) |
| Measles Antibody Levels at 19 Months of Age | Measles antibody levels were measured by haemagglutination inhibition assay (HAI) at 19 months of age, 4 weeks after MV challenge at 18 months of age. The aim was to determine whether the vaccination schedule at 9 months of age altered responses to a subsequent MV challenge at 18 months of age i.e., the antibody measurement was made 10 months after the first study intervention. | 10 months (19 months of age) |
| Background |
| Kristensen I, Aaby P, Jensen H. Routine vaccinations and child survival: follow up study in Guinea-Bissau, West Africa. BMJ. 2000 Dec 9;321(7274):1435-8. doi: 10.1136/bmj.321.7274.1435. |
| 15082643 | Background | Aaby P, Jensen H, Gomes J, Fernandes M, Lisse IM. The introduction of diphtheria-tetanus-pertussis vaccine and child mortality in rural Guinea-Bissau: an observational study. Int J Epidemiol. 2004 Apr;33(2):374-80. doi: 10.1093/ije/dyh005. |
| 15629363 | Background | Veirum JE, Sodemann M, Biai S, Jakobsen M, Garly ML, Hedegaard K, Jensen H, Aaby P. Routine vaccinations associated with divergent effects on female and male mortality at the paediatric ward in Bissau, Guinea-Bissau. Vaccine. 2005 Jan 19;23(9):1197-204. doi: 10.1016/j.vaccine.2004.02.053. |
| 15295708 | Background | Permar SR, Klumpp SA, Mansfield KG, Carville AA, Gorgone DA, Lifton MA, Schmitz JE, Reimann KA, Polack FP, Griffin DE, Letvin NL. Limited contribution of humoral immunity to the clearance of measles viremia in rhesus monkeys. J Infect Dis. 2004 Sep 1;190(5):998-1005. doi: 10.1086/422846. Epub 2004 Jul 26. |
| 9453614 | Background | Mills KH, Ryan M, Ryan E, Mahon BP. A murine model in which protection correlates with pertussis vaccine efficacy in children reveals complementary roles for humoral and cell-mediated immunity in protection against Bordetella pertussis. Infect Immun. 1998 Feb;66(2):594-602. doi: 10.1128/IAI.66.2.594-602.1998. |
| 12922130 | Background | Ovsyannikova IG, Reid KC, Jacobson RM, Oberg AL, Klee GG, Poland GA. Cytokine production patterns and antibody response to measles vaccine. Vaccine. 2003 Sep 8;21(25-26):3946-53. doi: 10.1016/s0264-410x(03)00272-x. |
| 11398113 | Background | Rowe J, Macaubas C, Monger T, Holt BJ, Harvey J, Poolman JT, Loh R, Sly PD, Holt PG. Heterogeneity in diphtheria-tetanus-acellular pertussis vaccine-specific cellular immunity during infancy: relationship to variations in the kinetics of postnatal maturation of systemic th1 function. J Infect Dis. 2001 Jul 1;184(1):80-8. doi: 10.1086/320996. Epub 2001 May 29. |
| 12270728 | Background | Lavigne MV, Castro M, Mateo N, Deluchi S, Atzori C, Piudo L, Calcagno M, Brero ML, Manghi M. Whole-cell Bordetella pertussis vaccine component modulates the mouse immune response to an unrelated soluble antigen. Microbes Infect. 2002 Jul;4(8):815-20. doi: 10.1016/s1286-4579(02)01601-5. |
| 15479435 | Background | Lindblad EB. Aluminium compounds for use in vaccines. Immunol Cell Biol. 2004 Oct;82(5):497-505. doi: 10.1111/j.0818-9641.2004.01286.x. |
| 9009322 | Background | Lindblad EB, Elhay MJ, Silva R, Appelberg R, Andersen P. Adjuvant modulation of immune responses to tuberculosis subunit vaccines. Infect Immun. 1997 Feb;65(2):623-9. doi: 10.1128/iai.65.2.623-629.1997. |
| 16782025 | Background | Weaver CT, Harrington LE, Mangan PR, Gavrieli M, Murphy KM. Th17: an effector CD4 T cell lineage with regulatory T cell ties. Immunity. 2006 Jun;24(6):677-688. doi: 10.1016/j.immuni.2006.06.002. |
| 15036232 | Background | Baecher-Allan C, Viglietta V, Hafler DA. Human CD4+CD25+ regulatory T cells. Semin Immunol. 2004 Apr;16(2):89-98. doi: 10.1016/j.smim.2003.12.005. |
| 15516964 | Background | Mills KH. Regulatory T cells: friend or foe in immunity to infection? Nat Rev Immunol. 2004 Nov;4(11):841-55. doi: 10.1038/nri1485. |
| 8193495 | Background | Smedman L, Joki A, da Silva AP, Troye-Blomberg M, Aronsson B, Perlmann P. Immunosuppression after measles vaccination. Acta Paediatr. 1994 Feb;83(2):164-8. doi: 10.1111/j.1651-2227.1994.tb13043.x. |
| 9744016 | Background | Pala S, Crimaldi G, Consolini R, Macchia P. [The cell-mediated response after measles vaccination]. Pediatr Med Chir. 1998 May-Jun;20(3):213-6. Italian. |
| 15032595 | Background | Sallusto F, Geginat J, Lanzavecchia A. Central memory and effector memory T cell subsets: function, generation, and maintenance. Annu Rev Immunol. 2004;22:745-63. doi: 10.1146/annurev.immunol.22.012703.104702. |
| 12690020 | Background | Aaby P, Bhuiya A, Nahar L, Knudsen K, de Francisco A, Strong M. The survival benefit of measles immunization may not be explained entirely by the prevention of measles disease: a community study from rural Bangladesh. Int J Epidemiol. 2003 Feb;32(1):106-16. doi: 10.1093/ije/dyg005. |
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| 25136102 | Background | Pulendran B. Systems vaccinology: probing humanity's diverse immune systems with vaccines. Proc Natl Acad Sci U S A. 2014 Aug 26;111(34):12300-6. doi: 10.1073/pnas.1400476111. Epub 2014 Aug 18. |
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| 25573105 | Background | Klein SL, Marriott I, Fish EN. Sex-based differences in immune function and responses to vaccination. Trans R Soc Trop Med Hyg. 2015 Jan;109(1):9-15. doi: 10.1093/trstmh/tru167. |
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| Protocol Violation |
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| Lost to Follow-up |
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| Physician Decision |
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| Poor Attendance |
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| BG001 | DTP + MV at 9 Months | DTP3 dose withheld and given HBV and OPV at four months of age. At 9 months of age given a single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid, and i.m. DTP (Serum Institute of India Ltd.) in the thigh. Yellow fever vaccine (YF) and OPV administered at 11 months of age. Given a standard MV challenge at 18 old. Measles Vaccine at 9 months of age: Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid at 9 months of age Diphtheria-tetanus-whole cell pertussis vaccine at 9 months of age: Single intramuscular (i.m.) dose of DTP (Serum Institute of India Ltd.) into the thigh at 9 months of age Measles Vaccine at 18 months of age: Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the de |
| BG002 | DTP at 9 Months | DTP3 dose withheld and given HBV and OPV at four months of age. At 9 months of age given i.m. DTP (Serum Institute of India Ltd.) in the thigh. MV, OPV and YF administered at 11 months of age. Given a standard MV challenge at 18 months of age. Diphtheria-tetanus-whole cell pertussis vaccine at 9 months of age: Single intramuscular (i.m.) dose of DTP (Serum Institute of India Ltd.) into the thigh at 9 months of age Measles Vaccine at 18 months of age: Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid at 18 months of age |
| BG003 | Total | Total of all reporting groups |
| days |
|
| Sex: Female, Male | Count of Participants | Participants |
|
| Race/Ethnicity, Customized | Count of Participants | Participants |
|
| Region of Enrollment | Number | participants |
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| OG001 | DTP + MV at 9 Months | DTP3 dose withheld and given HBV and OPV at four months of age. At 9 months of age given a single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid, and i.m. DTP (Serum Institute of India Ltd.) in the thigh. Yellow fever vaccine (YF) and OPV administered at 11 months of age. Given a standard MV challenge at 18 months old. Measles Vaccine at 9 months of age: Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid at 9 months of age Diphtheria-tetanus-whole cell pertussis vaccine at 9 months of age: Single intramuscular (i.m.) dose of DTP (Serum Institute of India Ltd.) into the thigh at 9 months of age Measles Vaccine at 18 months of age: Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the de |
| OG002 | DTP at 9 Months | DTP3 dose withheld and given HBV and OPV at four months of age. At 9 months of age given i.m. DTP (Serum Institute of India Ltd.) in the thigh. MV, OPV and YF administered at 11 months of age. Given a standard MV challenge at 18 months of age. Diphtheria-tetanus-whole cell pertussis vaccine at 9 months of age: Single intramuscular (i.m.) dose of DTP (Serum Institute of India Ltd.) into the thigh at 9 months of age Measles Vaccine at 18 months of age: Single standard intramuscular (i.m.) dose of measles vaccine (MV) (Edmonston Zagreb strain, Serum Institute of India Ltd., Pune, India) into the deltoid at 18 months of age |
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| Primary | Effect on CD4+FOXP3+CD127- Regulatory T Cell Frequencies | Regulatory T cell (Treg) frequencies (among all gated CD4+ T cells) at 9 and 10 months of age determined by flow cytometry and compared between the 3 intervention groups with the primary hypothesis that the MV+DTP group will have lower Treg frequencies than the MV group. | Study infants at 9 and 10 months of age. Not all participants had this assay done due to the follow reasons: failure to attend the appointment, unwell on the day of follow up, dropped out of the study, assay failure, insufficient blood sample volume or quality. | Posted | Mean | Standard Deviation | Percentage of CD4+ T cells | 4 weeks (9 months and 10 months of age) |
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| Secondary | Effect of Vaccine Group on Measles Antibody Levels | Effect of combining MV and DTP on measles antibody responses to see if combining vaccines interferes with antibody generation. Plasma measles antibodies measured by haemagglutination inhibition assay at 9 and 10 months of age. | Study infants at 9 and 10 months of age. Not all participants had this assay done due to the follow reasons: failure to attend the appointment, unwell on the day of follow up, dropped out of the study, assay failure, insufficient blood sample volume or quality. | Posted | Mean | Standard Deviation | Haemagglutination titre | 4 weeks (9 and 10 months of age) |
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| Secondary | Effect of Vaccine Group on Effector Memory T Cells (TEM) | CD4+CD45RO+CD62L- effector memory T cells measured by flow cytometry at 9 and 10 months of age | Study infants at 9 and 10 months of age. Not all participants had this assay done due to the follow reasons: failure to attend the appointment, unwell on the day of follow up, dropped out of the study, assay failure, insufficient blood sample volume or quality. | Posted | Mean | Standard Deviation | Percent of all CD4+ T cells | 4 weeks (9 and 10 months of age) |
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| Secondary | Effect of Vaccine Group on Diphtheria and Tetanus Antibody Levels | Plasma diphtheria and tetanus toxoid antibodies were measured by multiplex microsphere-based fluorescent immunoassay at 9 and 10 months of age. | Study infants at 9 and 10 months of age. Not all participants had this assay done due to the follow reasons: failure to attend the appointment, unwell on the day of follow up, dropped out of the study, assay failure, insufficient blood sample volume or quality. | Posted | Mean | Standard Deviation | IU/mL | 4 weeks (9 and 10 months of age) |
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| Secondary | Effect of Vaccine Group on Pertussis Toxoid Antibody Levels | Plasma pertussis toxoid antibodies were measured by multiplex microsphere-based fluorescent immunoassay at 9 and 10 months of age | Study infants at 9 and 10 months of age. Not all participants had this assay done due to the follow reasons: failure to attend the appointment, unwell on the day of follow up, dropped out of the study, assay failure, insufficient blood sample volume or quality. | Posted | Mean | Standard Deviation | EU/mL | 4 weeks (9 and 10 months of age) |
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| Secondary | Measles Antibody Levels at 19 Months of Age | Measles antibody levels were measured by haemagglutination inhibition assay (HAI) at 19 months of age, 4 weeks after MV challenge at 18 months of age. The aim was to determine whether the vaccination schedule at 9 months of age altered responses to a subsequent MV challenge at 18 months of age i.e., the antibody measurement was made 10 months after the first study intervention. | Study children at 19 months of age. Not all participants had this assay done due to the follow reasons: failure to attend the appointment, unwell on the day of follow up, dropped out of the study, assay failure, insufficient blood sample volume or quality. | Posted | Mean | Standard Deviation | HAI titre | 10 months (19 months of age) |
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| 106 |
| 1 |
| 106 |
| 88 |
| 106 |
| EG001 | DTP and MV Vaccination at 9 Months | Infants administered MV and DTP vaccines at 9 months of age | 2 | 115 | 0 | 115 | 90 | 115 |
| EG002 | DTP Vaccination at 9 Months | Infants administered DTP vaccine at 9 months of age | 0 | 81 | 0 | 81 | 66 | 81 |
| Chest infection | Respiratory, thoracic and mediastinal disorders | Systematic Assessment |
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| Rash, sores | Skin and subcutaneous tissue disorders | Systematic Assessment |
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| Diarrhoea and / or vomiting | Gastrointestinal disorders | Systematic Assessment |
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Not provided
Not provided
Not provided
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| Diphtheria toxoid antibodies at 9 months |
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| Diphtheria toxoid antibodies at 10 months |
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