Analysis of Lipid Profiles in Samples Sperm and Seminal Plasma Under Artificial Insemination

Analysis of Lipid Profiles in Samples Sperm and Seminal Plasma Under Artificial Insemination

Identify, by Lipidomics approaches, biochemical markers of fertility / infertility in sperm and seminal plasma linking the success or failure of the artificial insemination (IUI)

Analyze lipid profiles in samples of sperm and seminal plasma of three populations:

1. male sperm donors
2. men assigned to a program of assisted reproduction with normal sperm / slightly abnormal successfully in the IUI;
3. attached to a program of assisted reproduction with male semen Normal / slightly abnormal unsuccessfully in IUI

Establish partnerships between the composition of fatty acids, especially fatty acids polyunsaturated (PUFA) of the omega-3 (w3) series of samples analyzed parameterssemen and results of the IA to identify biochemical markers of fertility.

Assess markers found by checking the increase in the rate of success in the IA.

Inclusion Criteria Group A women donor group

• Fallopian unobstructed Men donor group
• Appear as sperm donor
• Age between 18-35 years
• Sexual abstinence 3-5 days

Exclusion Criteria Group A:

• Uterine malformations (fibroids, adenomyosis, uterine malformations or acquired congenital) and fallopian tubes (tubal occlusion)
• Obesity BMI (Body Mass Index)> 30 kg / m2
• Presence of altered tubal toxic habits (smoking> 15 cigarettes per day, alcohol or drug abuse).

Inclusión / Exclusion Criteria Group B and C:

• ≤ 38 years
• Natural cycles and stimulated
• The criteria inclusion / exclusion group A
• Time sterility not exceeding three years.