A New Micro Swim-up Procedure for Sperm Preparation in ICSI Treatment

The aim of this study is to evaluate the microbiological safety and the efficacy of a new not expensive, easy and fast technique (Direct Microswimup) for sperm preparation in In Vitro Fertilization (IVF) treatments without the use of centrifuge, able to simplify the procedure and to avoid mismatches by ensuring the same results of the standard techniques in terms of fertilization rate, blastulation rate, pregnancy/abortion rate, cryostorage rate, and a better euploid status of the embryos.

Inclusion Criteria:

• at least 8 oocytes retrieved at pickup
• oocyte insemination with ICSI technique
• only elective single embryo transfer (eSET) at blastocyst stage
• sperm concentration ≥ 1 X 106 /ml
• absence of antibiotic treatment in the previous four weeks for male patients

Exclusion Criteria:

• recurrent miscarriages
• more than three in vitro fertilization treatment
• azoospermia