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The investigators aim at exploring the differential/topographical in-situ expression of cytokines in the central nervous system (CNS) of patients who died with amyotrophic lateral sclerosis (ALS), using archived histopathology slides and residual paraffin blocks from autopsied cases. Previous studies from the investigators and other groups showed that inflammatory cytokines are implicated in several neurological affections, particularly neurodegenerative conditions. However, in-situ cytokine expression has never been studied so far in ALS. The investigators wanted to see if these neuro-mediators are involved in the neuromolecular chain/cascade underlying ALS.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Amyotrophic lateral sclerosis (ALS) | Archived/residual histopathology sections and paraffin blocks, retrieved entirely from residual autopsy material from patients who died with an amyotrophic lateral sclerosis. |
| |
| Control | Archived/residual histopathology sections and paraffin blocks, retrieved entirely from residual autopsy material from one non-ALS case. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| in-situ cytokine expression | Other |
|
| Measure | Description | Time Frame |
|---|---|---|
| in situ cytokine detection: interleukin 18 | IHC detection on histopathology sections of paraffin embedded brain tissue. | retrospective study performed on archived paraffin blocks, autopsies performed between 2000 and 2005 |
| in situ cytokine detection: interleukin 1 | IHC detection on histopathology sections of paraffin embedded brain tissue. | retrospective study performed on archived paraffin blocks, autopsies performed between 2000 and 2005 |
| in situ cytokine detection: interleukin 6 | IHC detection on histopathology sections of paraffin embedded brain tissue. | retrospective study performed on archived paraffin blocks, autopsies performed between 2000 and 2005 |
| in situ cytokine detection:TNF-a | IHC detection on histopathology sections of paraffin embedded brain tissue. | retrospective study performed on archived paraffin blocks, autopsies performed between 2000 and 2005 |
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Inclusion Criteria:
Exclusion Criteria:
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Archived/residual histopathology slides and paraffin blocks from residual autopsy material from patients who died with amyotrophic lateral sclerosis.
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| Name | Affiliation | Role |
|---|---|---|
| Hazim Kadhim, MD, PhD | CHU Brugmann | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| CHU Brugmann | Brussels | 1020 | Belgium |
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| ID | Term |
|---|---|
| D000690 | Amyotrophic Lateral Sclerosis |
| ID | Term |
|---|---|
| D013118 | Spinal Cord Diseases |
| D002493 | Central Nervous System Diseases |
| D009422 | Nervous System Diseases |
| D016472 | Motor Neuron Disease |
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Archived/residual histopathology sections and paraffin blocks entirely from residual autopsy material. Archived material involves old cases (autopsied between 2000 and 2005). All autopsies were conducted within the legal frame of the then applicable laws. There is no reference whatsoever to names or identifications of the deceased patients (anonymous samples).
| D019636 | Neurodegenerative Diseases |
| D057177 | TDP-43 Proteinopathies |
| D009468 | Neuromuscular Diseases |
| D057165 | Proteostasis Deficiencies |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |