Use of High Resolution Colonic Manometry in Studying Motility

Safety and Efficacy of High Resolution Manometry in Studying Colonic Motility and Iontophoretic Administration of Prokinetic Agents

An injury to the spinal cord (SCI) results in numerous medical complications, including gastrointestinal (GI) function. Individuals with SCI may experience severe constipation (prolonged stool retention), incontinence (accidents), and overall difficulty in bowel evacuation. While various treatments and medications have been established to address this complication, there is little knowledge relating to the overall colonic motility. However, new technology such as High Resolution Colonic Manometry allows physicians to visualize high pressure contractions inside the colon, leading to a greater understanding of typical motility, a more accurate diagnosis, and an effective treatment. In this study, the investigators propose to use this technology to understand the effects of SCI on colonic motility by comparing to normal colonic function observed in able-bodied (AB) individuals. In addition, the investigators plan to study the effects of pharmacological treatments, such as neostigmine, on promoting peristaltic contractions in the colon as it is delivered across the skin.

The investigators data suggests that the fundamental consequence of SCI is a slowing of intestinal peristaltic activity, most likely as a result of down regulation of parasympathetic neural pathways. Furthermore, the investigators have effectively shown that measures involved in increase parasympathetic stimulation results in efficient bowel evacuation and improved bowel care. Such measures include the administration of neostigmine (NEO), which increases peristaltic contractions in the colon, resulting in predictable bowel evacuations. Given the cardiopulmonary side-effects of NEO, an ant-cholinergic known as Glycopyrrolate (GLY) is usually co-administered with NEO to reverse these side effects. However, the investigators knowledge regarding the effects of SCI on colonic motility is still primitive, mainly due to pervious technological disadvantages. New High Resolution Colonic Manometry systems (Medical Measurement Systems Dover, NH) simultaneously capture high amplitude pressure contractions (HAPCs) across the ascending, transverse, descending, and recto-sigmoid colon. Such capabilities allow for a much clearer display of pressure events compared to previous line tracing manometric systems and the direction of contractions are much easier to discern as well. To date, this technology has been an effective tool in diagnosing bowel disorders and complications, such as constipation in able-bodied individuals (AB). However, such studies have not been conducted in a SCI population. This technology also has the potential to capture the effects of NEO+GLY on improving colonic function.

Inclusion Criteria:

• Chronic SCI >1year
• Able-Bodied (non SCI)
• Undergoing Elective Colonoscopy
• Age 18-75 years.

Exclusion Criteria:

• Previous adverse reaction or hypersensitivity to electrical stimulation
• Known sensitivity to neostigmine or glycopyrrolate
• Persons with SCI who do not require additional bowel care or have "normal bowel function"
• Known hypersensitivity to neostigmine or glycopyrrolate
• History of mechanical obstruction of the intestine or urinary tract
• History of Myocardial infarction
• Uncontrollable Hypertension Defined by a blood pressure reading of 160/100 mmHg or higher
• Organ damage (heart & kidney) and/or transient ischemic attack-cerebral vascular accident as a result of hypertension
• Known past history of coronary artery disease or bradyarrythmia
• Active respiratory disease
• Known history of asthma during lifetime or recent (within 3 months) respiratory infections
• Adrenal insufficiency
• Diagnosed coagulopathy
• Any renal disease or dysfunction
• Potential for pregnancy
• Lactating/nursing females
• Slow-heart beat (HR<45 bpm)
• Lack of mental capacity to give consent
• Use of any antibiotic in the past 7 days
• Use of medications known to affect the respiratory system
• Use of medications known to alter airway caliber
• Concurrent participation in other clinical trials (within 30 days)