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| Name | Class |
|---|---|
| Bezmialem Vakif University | OTHER |
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Aim of study is to determine reactive oxygen products and antioxidant capacity with rosacea patients; to measure metalloproteinase level and inflammatory markers of immune response and investigate their corelation with demodex infestation and also investigate the role of photoaging and sebum secretion in rosacea.
Rosacea is a common and chronic inflammatory disease characterized by facial erythema, papule, pustule and telangiectasia. Ultraviolet, Demodex mite, oxidative stress and antioxidant system disorders are emphasized in increased inflammation seen in rosacea.
The aim of our study was to evaluate the status of oxidative stress, matrix metalloproteinase (MMP) and inflammatory markers in systemic circulation, their relationship with demodex density and investigate the role of photoaging and sebum secretion in rosacea.
Forty patients with rosacea and age, gender and skin phenotype matched 40 healthy volunteers were enrolled in the study. Reflectance confocal microscopy (RCM) was used to calculate facial demodex density. The number of demodex, follicle, the number of mite per follicle, the number of infested follicle and the number of mite per infested follicle were calculated with RCM.
Photoaging severity and facial sebum levels were also assessed. Total oxidant capacity (TOC), total antioxidant capacity (TAC), oxidative stress index (OSI), paraoxonase-1 (PON-1), aryl esterase (ARES), myeloperoxidase (MPO), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-9 (MMP-9) levels in venous blood were measured.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| H, Rosacea patients | Rosacea patients who were between the ages of 18-70, having no systemic illness and who were not smoking. Obtaining a blood sample; TOC, TAC, OSI, PON-1, ARES, MPO, TNF-α, IL-1β, MMP-1 and MMP-9 levels in venous blood were measured Reflectance confocal microscopy; 1mm2-sized 10 images were taken from right cheek and forehead; the number of demodex, follicle, the number of mite per follicle, the number of infested follicle and the number of mite per infested follicle were calculated with RCM. And photoaging severity were also assessed by using RCM. Sebum rate at forehead and right cheek were evaluated with sebumeter. Dermoscopic photoaging scale were assessed by using video dermoscopy. |
| |
| K, Healthy volunteers | Healthy volunteers who were compatible in terms of age, sex and skin phenotype, having no systemic illness and who were not smoking. Obtaining a blood sample; TOC, TAC, OSI, PON-1, ARES, MPO, TNF-α, IL-1β, MMP-1 and MMP-9 levels in venous blood were measured Reflectance confocal microscopy; 1mm2-sized 10 images were taken from right cheek and forehead; the number of demodex, follicle, the number of mite per follicle, the number of infested follicle and the number of mite per infested follicle were calculated with RCM. And photoaging severity were also assessed by using RCM. Sebum rate at forehead and right cheek were evaluated with sebumeter. Dermoscopic photoaging scale were assessed by using video dermoscopy. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Obtaining a blood sample | Other | Total oxidant capacity (TOC), total antioxidant capacity (TAC), oxidative stress index (OSI), paraoxonase-1 (PON-1), aryl esterase (ARES), myeloperoxidase (MPO), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-9 (MMP-9) levels in venous blood were measured. |
| Measure | Description | Time Frame |
|---|---|---|
| Severity of rosacea | Rosacea patients who refer to criteria will be evaluated throughout 4 months. |
| Measure | Description | Time Frame |
|---|---|---|
| The number of demodex of right cheek and forehead | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. | |
| The number of follicle of right cheek and forehead | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Measure | Description | Time Frame |
|---|---|---|
| Sebum levels of right cheek and forehead | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. | |
| Photoaging severity of face by using RCM and dermoscopy | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
Inclusion Criteria:
Exclusion Criteria:
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Rosacea patients who are between the ages of 18-70, who having no systemic illness and who were not smoking and who were not using any topical and systemic treatment for a month attended to the study. As a control group it was planned that healthy volunteers who were compatible in terms of age, sex and skin phenotype, who were not smoking, with no systemic illness to be taken.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Tugba Falay | Contact | +905056545501 | tugbafalay@hotmail.com | |
| Vefa Aslı Erdemir | Contact | +905052685651 | vefaasli@hotmail.com |
| Name | Affiliation | Role |
|---|---|---|
| Tugba Falay | Istanbul Training and Research Hospital | Principal Investigator |
| Vefa Aslı Erdemir | Istanbul Training and Research Hospital | Study Director |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Istanbul Training and Research Hospital | Recruiting | Istanbul | Org. Abd. Nafiz Gürman | 34098 | Turkey (Türkiye) |
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| ID | Term |
|---|---|
| D012393 | Rosacea |
| ID | Term |
|---|---|
| D012871 | Skin Diseases |
| D017437 | Skin and Connective Tissue Diseases |
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| Reflectance confocal microscopy | Device | 1mm2-sized 10 images were taken from right cheek and forehead; the number of demodex, follicle, the number of mite per follicle, the number of infested follicle and the number of mite per infested follicle were calculated with RCM. And photoaging severity were also assessed by using RCM. |
|
| Sebumeter | Device | Sebum rate at forehead and right cheek were evaluated with sebumeter |
|
| Video dermoscopy | Device | Dermoscopic photoaging scale were assessed by using video dermoscopy. |
|
| The number of mite per follicle of right cheek and forehead | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| The number of infested follicle of right cheek and forehead | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| The number of mite per infested follicle of right cheek and forehead | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Total oxidant capacity (TOC) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Total antioxidant capacity (TAC) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Oxidative stress index (OSI) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Paraoxonase-1 (PON-1) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Aryl esterase (ARES) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Myeloperoxidase (MPO) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Tumor necrosis factor-alpha (TNF-α) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Interleukin-1beta (IL-1β) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Matrix metalloproteinase-1 (MMP-1) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Matrix metalloproteinase-1 (MMP-9) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Mehmet Salih Gürel |
| Istanbul Training and Research Hospital |
| Study Director |
| Abdurrahim Kocyigit | Bezmialem Vakif University | Study Director |
| Duygu Erdil | Istanbul Training and Research Hospital | Study Chair |
| Eray Metin Güler | Bezmialem Vakif University | Study Chair |