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| Name | Class |
|---|---|
| Société Nationale Française de Gastroentérologie | OTHER |
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Visceral hypersensitivity, low grade inflammation and increased intestinal permeability are three main pathophysiological mechanisms involved in irritable bowel syndrome. The connexion between these abnormalities is not known. We hypothesis there is a link between them in IBS with diarrhoea.
Irritable bowel syndrome (IBS) is a common functional disorder which affect around 10% of the general population. Abdominal pain and discomfort are associated with transit disorders (diarrhea, constipation, alternating). IBS is defined by Rome III criteria.
For clinicians, IBS remains difficult to treat while its pathophysiology remains not completely understood. Visceral hypersensitivity, low grade inflammation and increased intestinal permeability are three abnormalities found in IBS patients. Visceral hypersensitivity is present in 60% of the patients, while intestinal permeability is increased in a subgroup of IBS with diarrhea. Low grade inflammation could be identify with faecal calprotectin dosage. The link between this three abnormalities is not clear.
The goal of our study is to describe the prevalence of these three abnormalities in IBS-Diarrhea population and to look for a correlation between low grade inflammation, visceral hypersensitivity, increased intestinal permeability and clinical phenotypes.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Irritable Bowel Syndrome with diarrhea | Experimental | Colonoscopy with eleven biopsies in the left colon to assess intestinal permeability. Intestinal permeability is not routinely performed and is assessed in colonic biopsies (occludin, claudin and ZO-1 by western blot, qPCR and immunofluorescence) |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Colonoscopy with eleven biopsies in the left colon to assess intestinal permeability | Procedure | Eleven colonic biopsies are taken in the left colon during colonoscopy. Intestinal permeability is assessed by western blot, qPCR and immunofluorescence for claudin, occludin and ZO-1. |
| Measure | Description | Time Frame |
|---|---|---|
| Occludin level expression in left colonic biopsies | Occludin expression is measured using western blot (for proteins), Quantitative Real Time Polymerase Chain Reaction (q RT-PCR) (RNA) and IF (for localization) | day 1 |
| Faecal calprotectin level | Level is assessed on stool sample by ELISA kit in µg/g | day 1 |
| Measure | Description | Time Frame |
|---|---|---|
| Claudin level expression in left colonic biopsies | Claudin expression is measured using western blot (for proteins), q RT-PCR (for RNA) and IF (for localization) | day 1 |
| Zonula Occludens (ZO)-1 level expression |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Chloé Melchior, MD | University Hospital, Rouen | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Rouen University Hospital | Rouen | 76031 | France |
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|
ZO-1 expression is measured using western blot (for proteins), q RT-PCR (for RNA) and IF (for localization)
| day 1 |
| Pression pain threshold by rectal barostat | Pression pain threshold is measured in mmHg during rectal barostat. | day 1 |
| Quality of life by GIQLI | Evaluation of quality of life using the validated score : french version of the Gastrointestinal Quality of Life Index (GIQLI) | day 1 |
| Abdominal symptoms | Abdominal symptoms of IBS are assessed with IBS symptom severity scale. | day 1 |
| Anxiety and depression level | Anxiety and depression are assessed with the Hospital anxiety and depression scale. | day 1 |
| ID | Term |
|---|---|
| D043183 | Irritable Bowel Syndrome |
| ID | Term |
|---|---|
| D003109 | Colonic Diseases, Functional |
| D003108 | Colonic Diseases |
| D007410 | Intestinal Diseases |
| D005767 | Gastrointestinal Diseases |
| D004066 | Digestive System Diseases |
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| ID | Term |
|---|---|
| D003113 | Colonoscopy |
| ID | Term |
|---|---|
| D016099 | Endoscopy, Gastrointestinal |
| D016145 | Endoscopy, Digestive System |
| D003938 | Diagnostic Techniques, Digestive System |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
| D004724 | Endoscopy |
| D003949 | Diagnostic Techniques, Surgical |
| D013505 | Digestive System Surgical Procedures |
| D013514 | Surgical Procedures, Operative |
| D019060 | Minimally Invasive Surgical Procedures |
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